Izvorni znanstveni članak

Reproducibility, accuracy and concordance of Accutrend® Plus for measuring circulating lipid concentration in adults

Aldo Scafoglieri ; Department of Experimental Anatomy (EXAN), Vrije Universiteit Brussel, Brussels, Belgium
Jonathan Tresignie ; Department of Experimental Anatomy (EXAN), Vrije Universiteit Brussel, Brussels, Belgium
Steven Provyn ; Department of Experimental Anatomy (EXAN), Vrije Universiteit Brussel, Brussels, Belgium and Department of Anatomy, Morphology and Biomechanics, Haute Ecole Paul Henri Spaak, Brussels, Belgium
Jan Pieter Clarys ; Department of Experimental Anatomy (EXAN), Vrije Universiteit Brussel, Brussels, Belgium
Ivan Bautmans orcid.org/0000-0002-6820-9586 ; Frailty in Ageing research department (FRIA), Vrije Universiteit Brussel, Brussels, Belgium

Sažetak

Introduction: The determination of lipid biomarkers by capillary sampling may be useful in the scree-ning, diagnosis and/or personal management of hyperlipidemia and cardiovascular risk. It remains unclear whether the use of the Accutrend® Plus system is appropriate. This study aimed to assess its reproducibility, accuracy and concordance for blood lipid profiling in adults.
Materials and methods: Fasting capillary total cholesterol (TC) and triglyceride (TG) concentration on Accutrend® Plus were compared with their venous analogues obtained by a laboratory reference method in sixty-one adults (27 men and 34 women, aged 33.0 years). Supplementary capillary sam-pling was performed at two consecutive days taking into account macro-nutrient intake.
Results: The day-to-day reproducibility of the Accutrend® Plus system proved to be high for TC (ICC = 0.85, P < 0.001), but moderate for TG (ICC = 0.68, P < 0.001). Strong correlations (r ≥ 0.80, P < 0.001) with the reference method were found for TC and TG. Mean difference (limits of agreement) were: 0.26 mmol/L (-0.95, 1.47) for TC, and -0.16 mmol/L (-1.29, 0.98) for TG. The concordance for subject classification according to the National Cholesterol Education Program (NCEP) guidelines was significant (P < 0.001), with substantial agreement for TC (κw = 0.67), and moderate agreement for TG (κw = 0.50).
Conclusions: Day-to-day reproducibility of the Accutrend® Plus device for TC and TG is not optimal and lacks accuracy when compared to the reference laboratory method. The concordance between both methods for classifying subjects according to the NCEP is inadequate. Accutrend® Plus device should not be interchangeably used as a substitution for the standard laboratory methods in the diag-nosis of hyperlipidemia.

Ključne riječi

reproducibility of results; cholesterol; triglycerides

Hrčak ID:

77403

URI

https://hrcak.srce.hr/77403

Datum izdavanja:

15.2.2012.

Posjeta: 2.466 *