Veterinarski arhiv, Vol. 82 No. 1, 2012.
Kratko priopćenje
Characterization of infectious bursal disease virus field strains in Jordan using molecular techniques.
Dergham A. Roussn
; Provimi, Technical Department, Amman Jordan
Walid Al-Saleh
; CEVA Sante Animale, Poultry Range Technical Manager, Middle East Area, Jordan
Ghassan Y. Khawaldeh
; Provimi, Technical Department, Amman Jordan
Waheed S. Totanji
; Provimi, Technical Department, Amman Jordan
Sažetak
The reverse transcriptase-polymerase chain reaction/restriction fragment length polymorphism (RTPCR/ RFLP) and RT-PCR specific primer to detect very virulent (vv) strains of infectious bursal disease virus (IBDV) were used for identification and characterization of Jordanian field isolates of IBDV that caused severe outbreaks. In this study, 80 bursa of fabricius samples were used from 20 commercial broiler chicken flocks in Jordan with clinical symptoms of IBDV. The RT-PCR/RFLP was conducted on a 743-bp fragment of the VP2 gene with the restriction enzymes BstNI and MboI. The results indicate the existence of IBDV field strains in Jordan. In addition, 60% of IBDV Jordanian isolates had unique RFLP patterns different from those previously published elsewhere. However, 20% of local IBDV strains were positive on using a specific primer for vvIBDV and had a unique RFLP pattern that differed from the Jordanian IBDV isolate and those previously published elsewhere.
Ključne riječi
broiler; Jordan; infectious bursal disease; restriction fragment length polymorphism
Hrčak ID:
77415
URI
Datum izdavanja:
6.2.2012.
Posjeta: 1.740 *