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Veterinarski arhiv, Vol. 82 No. 2, 2012.

Izvorni znanstveni članak

Characterization of Vibrio alginolyticus isolated from white leg shrimp (Litopenaeus vannamei) with emphasis on its antibiogram and heavy metal resistance pattern.

Lee Seong Wei orcid id orcid.org/0000-0002-4854-2621 ; Department of Agro Industry, Faculty of Agro Industry and Natural Resources, Universiti Malaysia Kelantan, Campus Jeli, Kelantan, Malaysia
Wee Wendy orcid id orcid.org/0000-0002-4399-8937 ; Department of Fisheries Science, Faculty of Fisheries and Aqua-Industry, Universiti Malaysia Terengganu,Kuala Terengganu, Terengganu, Malaysia

Puni tekst: engleski pdf 171 Kb

str. 221-227

preuzimanja: 813

citiraj

Sažetak

This paper describes the antibiogram and heavy metal resistancepattern of Vibrio alginolyticus isolated from farmed white leg shrimp (Litopenaeus vannamei). V. alginolyticus has been recognized as a causative agent of vibriosis in shrimp culture and can devastate an entire shrimp farm. Therefore, this study was carried out to investigate the suitability of using antibiotics against V. alginolyticus, as well as its tolerance to heavy metals, to reveal the exposure level of cultured shrimp to heavy metals. In the present study, a total of 14 antibiotics; oxolinic acid (2 μg), ampicillin (10 μg), erythromycin (15 μg), furazolidone (15 μg), lincomycin (15 μg), colistin sulphate (25 μg), oleandomycin (15 μg), doxycycline (30 μg), fosfomycin (50 μg), florfenicol (30 μg), flumequine (30 μg), tetracycline (30 μg), fosfomycin (50 μg) and spiramycin (100 μg) as well as four heavy metals; mercury (Hg2+), cadmium (Cd2+), chromium (Cr6+) and copper (Cu2+) were applied. Based on the results for antibiotic sensitivity, it was shown that oxolinic acid is the most effective antibiotic in controlling V. alginolyticus, where 92.8% of the bacterial isolates present were sensitive to it. It was followed by furazolidone (85.6%), nitrofuratoin (79.8%), tetracycline (78.8%), doxycycline (72.2%) and florfenicol (71.2%). On the other hand, all the bacterial isolates present were resistant to lincomycin. In the heavy metal tolerance test, all the bacterial isolates present were resistant to Hg2+, Cd2+ and Cr6+ whereas only 26.7% of them were resistant to Cu2+.

Ključne riječi

antibiogram; heavy metal; Vibrio alginolyticus; white leg shrimp; Litopenaeus vannamei

Hrčak ID:

78275

URI

https://hrcak.srce.hr/78275

Datum izdavanja:

8.3.2012.

Podaci na drugim jezicima: hrvatski

Posjeta: 2.125 *