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Assessment of genetic diversity of wheat genotypes using microsatellite markers

VALENTINA ŠPANIĆ orcid id ; Agricultural Institute Osijek, Južno predgrađe 17, 31000 Osijek, Croatia
HERMANN BUERSTMAYR ; Department for Agrobiotechnology – IFA-Tulln, Institute of Biotechnology in Plant Production, BOKU – University of Natural Resources and Applied Life Sciences, Konrad Lorenz Str. 20. A-3430 Tulln. Austria
GEORG DREZNER ; Agricultural Institute Osijek, Južno predgrađe 17, 31000 Osijek, Croatia

Puni tekst: engleski pdf 211 Kb


str. 37-42

preuzimanja: 2.450



Background and Purpose: Genetic diversity is the material basis for crop improvement. In this study, genetic diversity of 30wheat genotypeswas evaluated at theDNAlevel using 24 simple sequence repeat (SSRs) markers. Materials and Methods: DNA extraction was performed according to the modified CTAB-method. Microsatellite analysis was performed using fluorescent fragment detection on a LI-COR 4200 DNA. Results and Discussion: The number of alleles per locus ranged from 1 to 14 with an average number of 8.44 alleles per locus. The highest number of alleles per locus was detected in the genome A with 7.2, compared to 5.9 and 5.0 for genomes B and D, respectively. The highest number of alleles was recorded at chromosome 7 (9.5), while the lowest number of alleles was detected at chromosomes 3 and 4 (5.0 and 5.3). The smallest genetic distance characterized genotypes Super Zitarka and Zitarka, Tena and Osjecanka, Tena and Bezostaja, Lela and Toras, Janica and Alka, Felix and Seka.Genotypes Pipi and Courtot showed the least genetic similiarities with rest of the genotypes. Conclusions: The identification of genetic diversity should be a good tool of selecting genotypes in breeding programs.

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