Izvorni znanstveni članak
Clinical and pathological tools for identifying microsatellite instability in colorectal cancer
Zoran Krivokapić
; Clinic for Digestive Surgery, Clinical Center of Serbia, Belgrade, Serbia
Srdjan Marković
; Department for Gastroenterology and Hepatology, Zvezdara University Clinical Center, Belgrade, Serbia
Jadranka Antić
; Institute of Endocrinology, Genetic laboratory, Clinical Center of Serbia, Belgrade, Serbia
Ivan Dimitrijević
; Clinic for Digestive Surgery, Clinical Center of Serbia, Belgrade, Serbia
Daniela Bojić
; Medical Faculty, University of Belgrade, Belgrade, Serbia
Petar Svorcan
; Medical Faculty, University of Belgrade, Belgrade, Serbia
Njegica Jojić
; Department for Gastroenterology and Hepatology, Zvezdara University Clinical Center, Belgrade, Serbia
Svetozar Damjanović
; Institute of Endocrinology, Genetic laboratory, Clinical Center of Serbia, Belgrade, Serbia
Sažetak
Aim To assess practical accuracy of revised Bethesda criteria
(BGrev), pathological predictive model (MsPath), and
histopathological parameters for detection of high-frequency
of microsatellite instability (MSI-H) phenotype in
patients with colorectal carcinoma (CRC).
Method Tumors from 150 patients with CRC were analyzed
for MSI using a fluorescence-based pentaplex polymerase
chain reaction technique. For all patients, we evaluated
age, sex, family history of cancer, localization, tumor
differentiation, mucin production, lymphocytic infiltration
(TIL), and Union for International Cancer Control stage.
Patients were classified according to the BGrev, and the
groups were compared. The utility of the BGrev, MsPath,
and clinical and histopathological parameters for predicting
microsatellite tumor status were assessed by univariate
logistic regression analysis and by calculating the sensitivity,
specificity, and positive (PPV) and negative (NPV) predictive
values.
Results Fifteen out of 45 patients who met and 4 of 105
patients who did not meet the BGrev criteria had MSI-H
CRC. Sensitivity, specificity, PPV, and NPV for BGrev were
78.9%, 77%, 30%, and 70%, respectively. MSI histology (the
third BGrev criterion without age limit) was as sensitive as
BGrev, but more specific. MsPath model was more sensitive
than BGrev (86%), with similar specificity. Any BGrev criterion
fulfillment, mucinous differentiation, and right-sided
CRC were singled out as independent factors to identify
MSI-H colorectal cancer.
Conclusion The BGrev, MsPath model, and MSI histology
are useful tools for selecting patients for MSI testing.
Ključne riječi
Hrčak ID:
85824
URI
Datum izdavanja:
15.8.2012.
Posjeta: 1.377 *