Veterinarski arhiv, Vol. 82 No. 5, 2012.
Izvorni znanstveni članak
Cell content in milk from cows with S. aureus intramammary infection.
Miroslav Benić
orcid.org/0000-0001-7594-520X
; Croatian Veterinary Institute, Zagreb, Croatia
Boris Habrun
; Croatian Veterinary Institute, Zagreb, Croatia
Gordan Kompes
; Croatian Veterinary Institute, Zagreb, Croatia
Željko Mihaljević
; Croatian Veterinary Institute, Zagreb, Croatia
Željko Cvetnić
; Croatian Veterinary Institute, Zagreb, Croatia
Marijan Cergolj
; Reproduction and Obstetrics Clinic, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Nino Maćešić
; Reproduction and Obstetrics Clinic, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Sažetak
Somatic cell count, viability of cells and percentages of the cell subsets (CD45, granulocytes, monocytes/macrophages, CD4, CD8 and B lymphocytes) were determined in milk from uninfected cow udder quarters (n = 7) and from quarters infected with S. aureus (n = 18). Cell subpopulations were double labelled with monoclonal antibodies and determined by flow cytometry. Percentages of epithelial cells, polymorphonuclear granulocytes, monocytes and lymphocytes were determined by light microscopy in stained smears. The SCC, percentages of cells labelled with antibodies specific to CD45, granulocytes, CD4 and CD8 epitope were statistically different between infected and uninfected quarters. The percentages of cells obtained by light microscopy were statistically different between the infected and uninfected groups. The percentages of granulocytes in infected animals were associated with the age and gravidity of the animals. Within the infected group, the percentage of CD8 varied statistically between imported replacement cows and animals raised on the farm. Values of epithelial cells and CD45- were moderately correlated (Pearson’s r = 0.47). The percentages of granulocytes
obtained by the two methods were also moderately correlated (Pearson’s r = 0.52).
Ključne riječi
milk; leukocytes; intramammary infection; S. aureus
Hrčak ID:
86239
URI
Datum izdavanja:
11.9.2012.
Posjeta: 1.298 *