Izvorni znanstveni članak
https://doi.org/10.2478/10004-1254-63-2012-2173
Cadmium and Zinc Induced Similar Changes in Protein and Glycoprotein Patterns in Tobacco (Nicotiana tabacum L.) Seedlings and Plants
Petra Peharec Štefanić
; Department of Molecular Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia
Sandra Šikić
; University of Zagreb, Zagreb, Croatia1, Department of Ecology Institute of Public Health, Zagreb, Croatia
Petra Cvjetko
; Department of Molecular Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia
Biljana Balen
orcid.org/0000-0002-3745-9865
; Department of Molecular Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia
Sažetak
The effects of 10 μmol L-1 and 15 μmol L-1 cadmium (Cd), a nonessential toxic element and 25 μmol L-1 and 50 μmol L-1 zinc (Zn), an essential micronutrient, on proteins and glycoproteins of Nicotiana tabacum L. seedlings and plants were investigated after exposure to each metal alone or to their combinations. Changes in only few polypeptides related to heavy metal treatments were observed in tobacco seedlings and leaves of adult plants, while the greatest change in total soluble protein pattern was observed in plant roots. Differences between control and treated tobacco tissues were more pronounced in the glycoprotein pattern, which was analysed by application of different lectins. The majority of the detected glycoproteins in leaves and roots of adult plants can be considered as a result of enhanced glycosylation due to heavy metal stress. The difference in glycoproteins between Cd and Zn application on tobacco seedlings and adult plants could not be determined since enhanced glycosylation was noticed after treatment with either metal alone or in combination. Therefore, it can be concluded that both metals induced N- and Oglycosylation as a result of changed environmental conditions.
Ključne riječi
heavy metals; lectins; protein pattern
Hrčak ID:
86890
URI
Datum izdavanja:
25.9.2012.
Posjeta: 2.346 *