Izvorni znanstveni članak
STR-typing of ancient skeletal remains: which multiplex-PCR kit is the best?
Melanie Harder
; Graduate School “Human Development in Landscapes”, Christian-Albrechts-University, Kiel, Germany
Rebecca Renneberg
; State criminal investigation department of Lower Saxony, Kiel Germany
Patrick Meyer
; Institute of Legal Medicine University Hospital of Schleswig- Holstein, Kiel, Germany,
Ben Krause-Kyora
; Institute of Clinical Molecular Biology, Christian-Albrechts-University, Kiel, Germany
Nicole von Wurmb-Schwark
; Graduate School “Human Development in Landscapes”, Christian-Albrechts-University, Kiel, Germany
Sažetak
Aim To comparatively test nine co mmercially available
short tandem repeat (STR)-multiplex kits (PowerPlex 16,
16HS, ES, ESI17, ESX17, S5 [all Promega]; AmpFiSTR Identifiler,
NGM and SEfiler [all Applied Biosystems]) for their efficiency
and applicability to analyze ancient and thus highly
degraded DNA samples.
Methods Fifteen human skeletal remains from the late
medieval age were obtained and analyzed using the nine
polymerase chain reaction assays with slightly modified
protocols. Data were systematically compared to find the
most meaningful and sensitive assay.
Results The ESI, ESX, and NGM kits showed the best overall
results regarding amplification success, detection rate,
identification of heterozygous alleles, sex determination,
and reproducibility of the obtained data.
Conclusion Since application of these three kits enables
the employment of different primer sequences for all the
investigated amplicons, a combined application is recommended
for best possible and – most importantly – reliable
genetic analysis of ancient skeletal material or otherwise
highly degraded samples, eg, from forensic cases.
Ključne riječi
Hrčak ID:
94933
URI
Datum izdavanja:
15.10.2012.
Posjeta: 1.431 *