Izvorni znanstveni članak
Evaluation of forensic and anthropological potential of D9S1120 in Mestizos and Amerindian populations from Mexico
Héctor Rangel-Villalobos
; Institute of Molecular Genetics Research, University of Guadalajara Ocotlán, Jalisco, Mexico
Viviana M Sánchez-Gutiérrez
; Institute of Molecular Genetics Research, University of Guadalajara Ocotlán, Jalisco, Mexico
Miriam Botello-Ruiz
; Institute of Molecular Genetics Research, University of Guadalajara Ocotlán, Jalisco, Mexico
Joel Salazar-Flores
; Institute of Molecular Genetics Research, University of Guadalajara Ocotlán, Jalisco, Mexico
Gabriela Martínez-Cortés
; Institute of Molecular Genetics Research, University of Guadalajara Ocotlán, Jalisco, Mexico
José F Muñoz-Valle
; Department of Molecular Biology and Genomics, University of Guadalajara, Guadalajara, Jalisco, Mexico
Christopher Phillips
; Forensic Genetics Unit, Institute of Legal Medicine, University of Santiago de Compostela, Santiago de Compostela, Spain
Sažetak
Aim To carry out a deeper forensic and anthropological
evaluation of the short tandem repeat (STR) D9S1120 in
five Mestizo populations and eight Amerindian groups
from Mexico.
Methods We amplified the STR D9S1120 based on primers
and conditions described by Phillips et al, followed by
capillary electrophoresis in the genetic analyzer ABI Prism
310. Genotypes were analyzed with the GeneMapper ID
software. In each population we estimated statistical parameters
of forensic importance and Hardy-Weinberg
equilibrium. Heterozygosity and FST-values were compared
with those previously obtained with nine STRs of the Combined
DNA Index System (CODIS-STRs).
Results Amerindian and Mestizo populations showed
high frequencies of the allele 9 and 16, respectively. Population
structure analysis (AMOVA) showed a significant
differentiation between Amerindian groups (FST = 2.81%;
P < 0.0001), larger than between Mestizos (FST = 0.44%;
P = 0.187). D9S1120 showed less genetic diversity but better
population differentiation estimates than CODIS-STRs
between Amerindian groups and between Amerindians
and Mestizos, but not between Mestizo groups.
Conclusion This study evaluated the ability of D9S1120
to be used for human identification purposes and demonstrated
its anthropological potential to differentiate Mestizos
and Amerindian populations
Ključne riječi
Hrčak ID:
94934
URI
Datum izdavanja:
15.10.2012.
Posjeta: 1.116 *