Izvorni znanstveni članak

https://doi.org/10.5562/cca2078

Protein and Glycoprotein Patterns Related to Morphogenesis in Horseradish Tissue Culture

Petra Peharec Štefanić ; Department of Molecular Biology, Faculty of Science, University of Zagreb, Horvatovac 102a, HR-10000 Zagreb, Croatia
Ana Smolko ; Department of Molecular Biology, Faculty of Science, University of Zagreb, Horvatovac 102a, HR-10000 Zagreb, Croatia
Anita Horvatić ; Ruđer Bošković Institute, Bijenička cesta 54, HR-10000 Zagreb, Croatia
Mario Cindrić ; Ruđer Bošković Institute, Bijenička cesta 54, HR-10000 Zagreb, Croatia
Marijana Krsnik-Rasol ; Department of Molecular Biology, Faculty of Science, University of Zagreb, Horvatovac 102a, HR-10000 Zagreb, Croatia
Biljana Balen orcid.org/0000-0002-3745-9865 ; Department of Molecular Biology, Faculty of Science, University of Zagreb, Horvatovac 102a, HR-10000 Zagreb, Croatia

Sažetak

In this study cellular proteins of in vitro grown horseradish (Armoracia lapathifolia Gilib.)
tissues of different morphologies, leaves (L), teratoma (TM) and tumor (TN), were separated by
one-dimensional (1-DE) and two-dimensional polyacrylamide gel electrophoresis (2-DE) in order to
reveal developmentally specific proteins. Moreover, proteins were subjected to analyses by lectin assays
to reveal whether the morphological differences were reflected in protein glycosylation. 1-DE glycoprotein
pattern showed substantial distinctions between horseradish tissues of different morphologies, which
was in contrast to silver stained protein profiles, where only slightly qualitative changes were observed.
2-DE revealed more differences in expression of cellular proteins between examined tissues in comparison
to 1-DE, while 2-DE blots confirmed quantitative difference in expression of 43 kDa glycoprotein
between investigated tissues. Mass spectrometry analysis identified this protein as a typical horseradish
peroxidase. Obtained results imply that the oligosaccharide structures could be important determinants of
specificities of horseradish tissue morphology. (doi: 10.5562/cca2078)

Ključne riječi

morphogenesis; horseradish; glycoproteins; lectine assay; tissue culture; 2-DE

Hrčak ID:

101905

URI

https://hrcak.srce.hr/101905

Datum izdavanja:

3.5.2013.

Posjeta: 2.662 *