Kratko priopćenje

https://doi.org/10.2478/acph-2013-0030

Development and validation of RP HPLC method for determination of betamethasone dipropionate in gingival crevicular fluid

LILJANA BOGDANOVSKA ; University “Ss Cyril and Methodius”, Faculty of Pharmacy, Skopje, Republic of Macedonia
MIRJANA POPOVSKA ; University “Ss Cyril and Methodius”, Faculty of Dentistry, Skopje, Republic of Macedonia
ANETA DIMITROVSKA ; University “Ss Cyril and Methodius”, Faculty of Pharmacy, Skopje, Republic of Macedonia
RUMENKA PETKOVSKA ; University “Ss Cyril and Methodius”, Faculty of Pharmacy, Skopje, Republic of Macedonia

Sažetak

A simple RP HPLC method for quantification of betamethasone dipropionate (BDP) in gingival crevicular fluid (GCF) has been developed and validated. GCF represents a valuable matrix for therapeutic monitoring of drugs used in the treatment of periodontal disease. The proposed method involves single step extraction for sample preparation. The calibration curve for BDP was linear over the concentration range of 0.1-2 µg mL–1 (R2 = 0.9971). RSD values of intra- and inter-day precision ranged 2.2–4.5 and 1.6–5.7 %, while accuracy values were higher than 96.6 and 97.0 %, respectively. The described method can be successfully applied for determination of betamethasone concentrations in GCF obtained from patients with chronic periodontitis after local treatment with BDP cream 0.5 mg g–1.

Ključne riječi

betamethasone dipropionate; gingival crevicular fluid; HPLC; periodontal disease

Hrčak ID:

102467

URI

https://hrcak.srce.hr/102467

Datum izdavanja:

30.9.2013.

Posjeta: 3.250 *