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Comparison of CE and HPLC Methods for Determining Lovastatin and Its Oxidation Products after Exposure to an Oxidative Atmosphere

Sandra Javernik Rajh ; Research & Development Division, Krka d.d., Šmarješka cesta 6, SI-8501 Novo mesto, Slovenia
Samo Kreft ; Department for Pharmacognosy, Faculty of Pharmacy, University of Ljubljana, Aškerčeva 7, SI-1000 Ljubljana, Slovenia
Borut Štrukelj ; Department for Pharmacognosy, Faculty of Pharmacy, University of Ljubljana, Aškerčeva 7, SI-1000 Ljubljana, Slovenia
Franc Vrečer ; Research & Development Division, Krka d.d., Šmarješka cesta 6, SI-8501 Novo mesto, Slovenia


Puni tekst: engleski pdf 135 Kb

str. 263-268

preuzimanja: 1.607

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Sažetak

Lovastatin is a cholesterol-lowering agent, which competitively inhibits the enzyme HMG-CoA reductase. Several HPLC methods for its analysis have been developed but there is no report of its determination using capillary electrophoresis (CE). In this paper, we report the development of a simple CE method for lovastatin determination, which is selective with respect to its degradation products and useful for routine analyses. Since the molecule of lovastatin in its lactone form is uncharged and is only slightly soluble in water, base hydrolysis was used to open the lactone ring and transform the compound into a water-soluble acid form, which is negatively charged. Different solvents, different amounts of NaOH added, different hydrolysis times and different temperatures for sample preparation were tested. The CE and HPLC methods are compared in terms of susceptibility, precision, linearity and accuracy. HPLC method was found to be more susceptible and more precise.

Ključne riječi

lovastatin, lovastatin acid, analytical method, capillary electrophoresis, CE, high performance liquid chromatography, HPLC

Hrčak ID:

103177

URI

https://hrcak.srce.hr/103177

Posjeta: 2.051 *