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Taurine attenuates oxidative stress and alleviates cardiac failure in type I diabetic rats

Guo-guang Wang ; Department of Pathophysiology Wannan Medical College, Wuhu, China
Wei Li ; Department of Pathophysiology Wannan Medical College, Wuhu, China
Xiao-hua Lu ; Department of Pathophysiology Wannan Medical College, Wuhu, China
Xue Zhao ; Department of Pathophysiology Wannan Medical College, Wuhu, China
Lei Xu ; Department of Biochemistry Wannan Medical College, Wuhu, China

Puni tekst: engleski pdf 537 Kb

str. 171-179

preuzimanja: 913



Aim To investigate cardioprotective effect of taurine in diabetic
Methods Male Sprague-Dawley rats were assigned randomly
into four groups of 15 rats: control group, control
+ taurine group, streptozotocin (STZ) group, and STZ +
taurine group. Rats in STZ and STZ+ taurine groups were
treated by a single injection of STZ (70 mg kg-1, intraperitoneally)
dissolved in 0.01 M citrate buffer (pH 4.5) for induction
of diabetes, and rats in control and control + taurine
groups were treated with the same volume citrate buffer.
Taurine was orally administered to rats in control + taurine
and STZ + taurine groups daily for 8 weeks. Rats were examined
for diabetic cardiomyopathy by left ventricular (LV)
hemodynamic analysis. Myocardial oxidative stress was assessed
by measuring the activity of superoxide dismutase
(SOD) and the level of malondialdehyde (MDA). Myocardial
protein kinase B (Akt/PKB) phosphorylation and heme oxygenase-
1 (HO-1) protein levels were measured by Western
blot in all rats at the end of the study.
Results In untreated diabetic rats, LV systolic pressure, rate
of pressure rise, and rate of pressure fall were decreased,
while LV end-diastolic pressure was increased, indicating
reduced LV contractility and slowing of LV relaxation. The
levels of Akt/PKB phosphorylation and SOD activity were
decreased and HO-1 protein expression and MDA content
increased. Taurine treatment significantly improved LV systolic
and diastolic function, and there were persistent increases
in activities of Akt/PKB and SOD, and the level of
HO-1 protein.
Conclusion Taurine treatment ameliorates myocardial
function and heart oxidant status, while increasing myocardial
Akt/PKB phosphorylation, and HO-1 levels have
beneficial effects on diabetic cardiomyopathy.

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