Izvorni znanstveni članak
https://doi.org/10.3325/cmj.2013.54.232
Genetic parameters and allele frequencies of five new European Standard Set STR loci (D10S1248, D22S1045, D2S441, D1S1656, D12S391) in the population of Romania
Florin Stanciu
; National Forensic Science Institute Bucharest, Romania
Simona Vladu
; National Forensic Science Institute Bucharest, Romania
Veronica Cuţăr
; National Forensic Science Institute Bucharest, Romania
Daniela Cocioabă
; National Forensic Science Institute Bucharest, Romania
Florentina Iancu
; National Forensic Science Institute Bucharest, Romania
Adnana Cotolea
; National Forensic Science Institute Bucharest, Romania
Ionel Marius Stoian
; National Forensic Science Institute Bucharest, Romania
Sažetak
Aim To establish allele frequencies and genetic parameters
for 5 new European Standard Set short tandem repeat
(STR) loci in the population of Romania and to compare
them with those in other populations.
Methods DNA was isolated using QIAamp 96 DNA Swab
BioRobot Kit and Chelex 100 methods. Polymerase chain reaction
amplification was done using Investigator ESSplex-
Plus Kit (D1S1656, D2S441, D2S1338, D3S1358, D8S1179,
D10S1248, D12S391, D16S539, D18S51, D19S433, D21S11,
D22S1045, FGA, TH01, and vWA). For DNA typing, Applied
Biosystems 3500/3500xL Genetic Analyzer was used. Statistical
analysis was done using Powerstats, GDA, and Arlequin
software.
Results Power of discrimination and polymorphism information
content was highest for two new ESS loci, D1S1656
and D12S391. Comparison of allele frequencies for 5 new
ESS loci in Romanian population with previously published
population data showed significant differences for
all compared populations, with the exception of Hungary.
Geographically more distant populations, such as Spain,
Sweden, United Kingdom, Germany, and Portugal differed
more than closer populations.
Conclusion New ESS STR loci are very useful for the analysis
of forensic samples (persons or traces) due to their characteristics
(shortness and high polymorphism). In comparisons
with other common STR markers, they have a higher
power of discrimination and also higher polymorphism information
content, and could be used in any national DNA
database.
Ključne riječi
Hrčak ID:
104760
URI
Datum izdavanja:
15.6.2013.
Posjeta: 1.888 *