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Analysis of 8 X-chromosomal markers in the population of central Croatia

Branka Gršković ; Forensic Science Centre “Ivan Vučetić,” General Police Directorate Ministry of Interior, Zagreb, Croatia
Anastassiya Zidkova ; Institute of Biology and Medical Genetics, First Faculty of Medicine Charles University in Prague and General University Hospital in Prague, Prague, Czech Republic
Vlastimil Stenzl ; Department of Forensic Genetics, Institute of Criminalistics, Prague, Czech Republic
Maja Popović ; University Center for Forensic Sciences, University of Split, Split, Croatia
Dragan Primorac ; University of Split, School of Medicine, Split, Croatia
Gordan Mršić ; Forensic Science Centre “Ivan Vučetić,” General Police Directorate Ministry of Interior, Zagreb, Croatia

Puni tekst: engleski pdf 260 Kb

str. 238-247

preuzimanja: 692



Aim To analyze 8 X-linked short tandem repeat (STR) markers
in the population of central Croatia and to evaluate
their forensic efficiency.
Methods We carried out a statistical analysis of the data
from previously performed genetic analyses, collected
during routine forensic work by the Forensic Science Centre
‘‘Ivan Vučetić.’’ Mentype® Argus X-8 PCR amplification kit
was used for typing the data of 99 unrelated healthy women
and 78 men from central Croatia. Haplotype frequencies
were calculated only in male samples. Arlequin 3.5
software was used to assess Hardy-Weinberg equilibrium
(HWE), linkage disequilibrium (LD), observed and expected
heterozygosity. Power of discrimination (PD) for men and
women, polymorphism information content (PIC), power
of exclusion, and mean exclusion chance for deficiency
cases, normal trios, and duos were determined using online
Results In female samples, deviations from HWE (P = 0.006)
for each locus were not found. LD test performed both
on female and male samples revealed no significant association
between markers (P = 0.002). DXS10135 was
the most polymorphic locus (PIC = 0.931). PD varied from
0.692 to 0.935 in male and from 0.845 to 0.992 in female
samples. Combined PD reached 99.999999% in men and
99.9999999999% in women.
Conclusion Performed analyses revealed that the studied
marker set contained polymorphic markers with high
power of discrimination. We can conclude that Mentype®
Argus X-8 PCR is suitable for application in the population
of central Croatia. Results of this study, together with collected
allele and haplotype frequencies, are the first step
in establishing a national reference X-STR database based
on 8 X-STR loci.

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