Izvorni znanstveni članak
https://doi.org/10.3325/cmj.2013.54.263
Effect of ultraviolet C radiation on biological samples
Branka Gršković
; Forensic Science Centre “Ivan Vučetić”, General Police Directorate Ministry of Interior, Zagreb, Croatia
Dario Zrnec
; Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia
Maja Popović
; University Center for Forensic Sciences, University of Split, Split, Croatia
Maja Jelena Petek
; Forensic Science Centre “Ivan Vučetić”, General Police Directorate Ministry of Interior, Zagreb, Croatia
Dragan Primorac
; University of Split School of Medicine, Split, Croatia
Gordan Mršić
; Forensic Science Centre “Ivan Vučetić”, General Police Directorate Ministry of Interior, Zagreb, Croatia
Sažetak
Aim To examine the influence of ultraviolet C (UVC) radiation
on blood, saliva, semen, and naked DNA samples for
preventing DNA cross-contamination on working surfaces
in laboratories.
Methods Blood, saliva, semen, and DNA isolated from buccal
swab samples were obtained from a single male donor
and applied to the laboratory working surfaces. UVC radiation
was applied to these diluted and undiluted samples
with or without previous decontamination of the working
surfaces with 10% sodium hypochlorite and 20% ethanol.
Genomic DNA was extracted using Chelex. After quantification,
DNA was amplified using the AmpFlSTR® NGM™
PCR Amplification Kit. We tested and statistically analyzed
DNA concentration, UVC dose, sample volume, radiation
time, the number of correctly detected alleles on genetic
loci, and the number of correctly detected alleles in four
groups in which 16 loci were divided.
Results When working surfaces were not decontaminated
and were treated only with UVC radiation in the laboratory,
the genetic profile for naked DNA could not be obtained
after 2 minutes of UVC radiation and for saliva after 54
hours. For blood and semen, a partial genetic profile was
obtained even after 250 hours of UVC radiation in the laminar.
When working surfaces were decontaminated with
10% sodium hypochlorite and 20% ethanol, genetic profile
could not be obtained for naked DNA after 2 minutes, for
saliva after 4 hours, for blood after 16 hours, and for semen
after 8 hours of UVC radiation in the laboratory.
Conclusion It is recommended to carefully and thoroughly
clean working surfaces with 10% sodium hypochlorite
and 20% ethanol followed by minimal 16-hour UVC exposure
(dose approximately 4380 mJ/cm2) for complete and
successful decontamination.
Ključne riječi
Hrčak ID:
104770
URI
Datum izdavanja:
15.6.2013.
Posjeta: 1.239 *