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https://doi.org/10.3325/cmj.2013.54.286

Haplotype data for 23 Y-chromosome markers in a reference sample from Bosnia and Herzegovina

Lejla Kovačević ; Faculty of Pharmacy, University in Sarajevo, Sarajevo, Bosnia and Herzegovina
Vera Fatur-Cerić ; Faculty of Pharmacy, University in Sarajevo, Sarajevo, Bosnia and Herzegovina
Negra Hadžić ; Institute for Genetic Engineering and Biotechnology, Sarajevo, Bosnia and Herzegovina
Jasmina Čakar ; Institute for Genetic Engineering and Biotechnology, Sarajevo, Bosnia and Herzegovina
Dragan Primorac ; University of Split, School of Medicine and Department of Forensic Science, Split, Croatia
Damir Marjanović ; Genos Ltd, Zagreb, Croatia


Puni tekst: engleski pdf 92 Kb

str. 286-290

preuzimanja: 645

citiraj


Sažetak

Aim To detect polymorphisms of 23 Y-chromosomal short
tandem repeat (STR) loci, including 6 new loci, in a reference
database of male population of Bosnia and Herzegovina,
as well as to assess the importance of increasing
the number of Y-STR loci utilized in forensic DNA analysis.
Methods The reference sample consisted of 100 healthy,
unrelated men originating from Bosnia and Herzegovina.
Sample collection using buccal swabs was performed in
all geographical regions of Bosnia and Herzegovina in the
period from 2010 to 2011. DNA samples were typed for 23
Y STR loci, including 6 new loci: DYS576, DYS481, DYS549,
DYS533, DYS570, and DYS643, which are included in the
new PowerPlex® Y 23 amplification kit.
Results The absolute frequency of generated haplotypes
was calculated and results showed that 98 samples had
unique Y 23 haplotypes, and that only two samples shared
the same haplotype. The most polymorphic locus was
DYS418, with 14 detected alleles and the least polymorphic
loci were DYS389I, DYS391, DYS437, and DYS393.
Conclusion This study showed that by increasing the
number of highly polymorphic Y STR markers, to include
those tested in our analysis, leads to a reduction of repeating
haplotypes, which is very important in the application
of forensic DNA analysis.

Ključne riječi

Hrčak ID:

104779

URI

https://hrcak.srce.hr/104779

Datum izdavanja:

15.6.2013.

Posjeta: 1.226 *