Izvorni znanstveni članak

https://doi.org/10.5562/cca2067

Double Amino Functionalized Ag Nanoparticles as SERS Tags in Raman Diagnostic

Simona Cîntă Pînzaru orcid.org/0000-0001-8016-4408 ; Department of Biomedical Physics, Theoretics and Molecular Spectroscopy, Babeş-Bolyai University, Kogălniceanu 1, RO-400084 Cluj-Napoca, România
Alexandra Falamaş ; Department of Biomedical Physics, Theoretics and Molecular Spectroscopy, Babeş-Bolyai University, Kogălniceanu 1, RO-400084 Cluj-Napoca, România
Cristina Dehelean ; Victor Babeş University of Medicine and Pharmacy, Faculty of Pharmacy, Eftimie Murgu Square 2, RO- 300041, Timişoara, România
Cristian Morari ; INCDTIM Cluj-Napoca, Donath Str. 65-103, 400239, Cluj-Napoca, Romania
Monica Venter ; Babeş-Bolyai University, Faculty of Chemistry and Chemical Engineering, Arany Janos 11, RO-400028 Cluj-Napoca, România

Sažetak

Surface enhanced Raman scattering (SERS) effect is currently exploited as the basis of a new type of optical labels for in vivo investigation of the tissues, especially for early medical diagnostic. Silver colloidal nanoparticles decorated with chemisorbed cresyl violet molecular species could act as hybrid SERS labels. Their Raman scattering properties have been characterized here using different SERS techniques, and probing different excitation wavelengths, even in the near infrared. Obtaining FT-SERS signal of cresyl violet is of particular importance for applying FT-Raman spectroscopy to the tissues or cells, in providing sensitive information in the close vicinity of the SERS label incubated into the biological sample. Furthermore, upon chemisorption on the silver nanoparticles, cresyl violet molecular orientation provided both amino functional groups free of interaction with the silver, resulting double amino-functionalized Ag nanoparticles suitable for DNA tagging. SERS tests of melanoma induced in mouse using CV-Ag SERS label suggested the possibility to setup the tissue labeling procedure for skin cancer monitoring.(doi: 10.5562/cca2067)

Ključne riječi

FT-SERS; SERS label; cresyl violet; amino functionalized-Ag nanoparticles

Hrčak ID:

111215

URI

https://hrcak.srce.hr/111215

Datum izdavanja:

20.11.2013.

Posjeta: 2.795 *