Izvorni znanstveni članak
Improvement of Enzymatic Hydrolysis of Steam-exploded Wheat Straw by Simultaneous Glucose and Xylose Liberation
M. Marcos
; Department of Chemical Engineering and Environmental Technology, University of Valladolid, Doctor Mergelina s/n, 47011 Valladolid, Spain
M. T. García-Cubero
; Department of Chemical Engineering and Environmental Technology, University of Valladolid, Doctor Mergelina s/n, 47011 Valladolid, Spain
G. González-Benito
; Department of Chemical Engineering and Environmental Technology, University of Valladolid, Doctor Mergelina s/n, 47011 Valladolid, Spain
M. Coca
; Department of Chemical Engineering and Environmental Technology, University of Valladolid, Doctor Mergelina s/n, 47011 Valladolid, Spain
S. Bolado
; Department of Chemical Engineering and Environmental Technology, University of Valladolid, Doctor Mergelina s/n, 47011 Valladolid, Spain
S. Lucas
; Department of Chemical Engineering and Environmental Technology, University of Valladolid, Doctor Mergelina s/n, 47011 Valladolid, Spain
Sažetak
This work aimed at enhancing enzymatic hydrolysis of steam-exploded wheat-straw by investigating factors affecting hydrolysis. A multi-objective optimization of glucose and xylose release was performed using Celluclast 1.5L and Ultraflo-L mixtures with maximal values of 20 for enzyme/substrate ratio, 72 h for reaction time, 50 °C for temperature and 5.0 for pH. The highest sugar yields obtained were 18.9 ± 0.4 g/100gDM for glucose and 4.7 ± 0.2 g/100gDM for xylose. The addition of Ultraflo-L could increase the liberation of xylose, but has no pronounced effect on glucose release. The effect of β-glucosidase addition to the Ultraflo+Celluclast mixture for improving sugar yield was also studied. The β-glucosidase supplementation increased the production by approximately 29.9 % for glucose and 5.9 % for xylose, when a β-glucosidase loading of 10 % gβ-glucosidase/gcellulose was used.
Ključne riječi
Enzymatic hydrolysis; cellulase; β-glucosidase; RSM; multiobjective optimization
Hrčak ID:
112378
URI
Datum izdavanja:
19.12.2013.
Posjeta: 1.477 *