Izvorni znanstveni članak
https://doi.org/10.3325/cmj.2014.55.10
Validation of 58 autosomal individual identification SNPs in three Chinese populations
Yi-Liang Wei
; Key Laboratory of Forensic Genetics, Institute of Forensic Science, Ministry of Public Security, Beijing, P.R. China
Cui-Jiao Qin
; Key Laboratory of Forensic Genetics, Institute of Forensic Science, Ministry of Public Security, Beijing, P.R. China
Hai-Bo Liu
; Institution of Forensic Science of Bingtuan Public Security Bureau Xinjiang, P.R. China
Jing Jia
; Key Laboratory of Forensic Genetics, Institute of Forensic Science, Ministry of Public Security, Beijing, P.R. China
Lan Hu
; Key Laboratory of Forensic Genetics, Institute of Forensic Science, Ministry of Public Security, Beijing, P.R. China
Cai-Xia Li
; Key Laboratory of Forensic Genetics, Institute of Forensic Science, Ministry of Public Security, Beijing, P.R. China
Sažetak
Aim To genotype and evaluate a panel of single-nucleotide
polymorphisms for individual identification (IISNPs)
in three Chinese populations: Chinese Han, Uyghur, and
Tibetan.
Methods Two previously identified panels of IISNPs, 86 unlinked
IISNPs and SNPforID 52-plex markers, were pooled
and analyzed. Four SNPs were included in both panels.
In total, 132 SNPs were typed on Sequenom MassARRAY®
platform in 330 individuals from Han Chinese, Uyghur, and
Tibetan populations. Population genetic indices and forensic
parameters were determined for all studied markers.
Results No significant deviation from Hardy-Weinberg
equilibrium was observed for any of the SNPs in 3 populations.
Expected heterozygosity (He) ranged from 0.144 to
0.500 in Han Chinese, from 0.197 to 0.500 in Uyghur, and
from 0.018 to 0.500 in Tibetan population. Wright’s Fst values
ranged from 0.0001 to 0.1613. Pairwise linkage disequilibrium
(LD) calculations for all 132 SNPs showed no
significant LD across the populations (r2<0.147). A subset
of 58 unlinked IISNPs (r2<0.094) with He>0.450 and Fst values
from 0.0002 to 0.0536 gave match probabilities of 10−25
and a cumulative probability of exclusion of 0.999992.
Conclusion The 58 unlinked IISNPs with high heterozygosity
have low allele frequency variation among 3 Chinese
populations, which makes them excellent candidates for
the development of multiplex assays for individual identification
and paternity testing.
Ključne riječi
Hrčak ID:
117030
URI
Datum izdavanja:
15.2.2014.
Posjeta: 1.266 *