Izvorni znanstveni članak
https://doi.org/10.3325/cmj.2014.55.14
Slovenian population data for five new European Standard Set short tandem repeat loci and SE33 locus
Irena Zupanič Pajnič
; Institute of Forensic Medicine Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia
Eva Podovšovnik Axelsson
; Turistica – Faculty of Tourism Studies, University of Primorska Portorož, Slovenia
Jože Balažic
; Institute of Forensic Medicine Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia
Sažetak
Aim To establish the allele distribution and statistical parameters
of forensic interest for the D10S1248, D22S1045,
D2S441, D1S1656, D12S391, and SE33 loci in Slovenian
population and to compare allele frequencies with those
from other populations.
Methods We analyzed blood and buccal swab samples
from 333 unrelated, healthy Slovenian individuals. All
samples were genotyped using the AmpFlSTR NGM Kit
to obtain the allele frequency data for the loci D10S1248,
D22S1045, D2S441, D1S1656, and D12S391. Samples from
113 individuals were also analyzed using the PowerPlex
ESX 17 system to obtain the allele frequency data for the
SE33 locus. Allele frequencies and statistical parameters of
forensic interest were determined and frequency profiles
compared between Slovenian and other European Caucasian
populations using the Arlequin software, version
3.5.1.3.
Results The investigated short tandem repeat (STR) loci in
Slovenian population had a great discriminating potential
with a combined discrimination power of 0.99999998. The
highest discrimination power and polymorphism information
content were observed for the SE33 locus, followed by
loci D1S1656, D12S391, D10S1248, D2S441, and D22S1045.
When Slovenian allele frequency distribution was compared
with other European populations, deviations were
found only for Spanish and Italian population for D2S441
and D12S391.
Conclusion Slovenian population does not differ significantly
from other European populations in terms of allele
frequency distributions for the six analyzed STR loci. Based
on forensic efficiency values, SE33 may be considered the
most informative locus, which makes it especially useful in
forensic investigations.
Ključne riječi
Hrčak ID:
117031
URI
Datum izdavanja:
15.2.2014.
Posjeta: 1.102 *