Izvorni znanstveni članak

CpG methylation analysis at the putative differential methylated region of the Xist gene in goats (Capra hircus) somatic cells.

Bikash R. Prusty orcid.org/0000-0002-1691-9068 ; Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly, India
Dharmendra Kumar ; Animal Biotechnology Centre, MPPCVV, Jabalpur, India
Tapan K. Palai ; Division of Animal Biochemistry, Indian Veterinary Research Institute, Izatnagar, Bareilly, India
Subas C. Jena ; Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly, India
Megha K. Bedekar ; Central Institute of Fisheries and Education, Mumbai, Maharashtra, India
Sanjeev Singh ; National Bureau of Animal Genetic Resources, Karnal, Haryana, India
Bikash C. Sarkhel ; Animal Biotechnology Centre, MPPCVV, Jabalpur, India

Sažetak

X chromosome inactivation is a complex epigenetic process that ensures equal expression of most Xlinked genes in both sexes. Xist (X-chromosome inactive specific transcript) is a crucial development related gene that initiates X chromosome inactivation in mammalian females during embryogenesis. DNA methylation is a major chromatin modification involved in transcriptional repression of genes, regulating X chromosome inactivation, genomic imprinting, and cell or tissue specific gene expression and in many other processes during embryo development. Appropriate DNA methylation of cytosine residues at CpG dinucleotide within the differential methylated region of many imprinted genes is required for normal post implantation development of a mammalian embryo. An aberrant pattern of DNA methylation because of incomplete or anomalous nuclear reprogramming of somatic cells is a reason for poor success rate of somatic cell nuclear transfer in farm animals. In the present study, a putative differential methylated region was identified for first time in goats (Capra hircus) followed by study of the DNA methylation status of this region, using bisulfite sequencing analysis taking fibroblast and cumulus cells from goats. The putative differential methylated region contains 10 CpG sites with a mean methylation level of 92% and 94% for fi broblast and cumulus cells respectively. Phylogenetic analysis of this region revealed goats and sheep positioned in a single cluster distinct from others studied. This study will be a definite help in future research on molecular events responsible for complete nuclear reprogramming of goat somatic cells in SCNT.

Ključne riječi

Xist gene; DNA methylation; epigenetic reprogramming; bisulfite sequencing; somatic cell nuclear transfer; goat

Hrčak ID:

125125

URI

https://hrcak.srce.hr/125125

Datum izdavanja:

16.7.2014.

Podaci na drugim jezicima: hrvatski

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