Izvorni znanstveni članak

Brinzolamide-induced retinopathy in neonatal rats: an alternative animal model of retinal neovascularization

Y. A. DYOMIN ; Kharkiv Medical Academy of Postgraduate Education
P.V. BILETSKA ; Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Cryomorphology department, 61001, Derzhavinska str. 2, 363, Kharkiv, Ukraine
I. D. GAPUNIN ; Specialized Clinic of Ukrainian Ministry of Health

Sažetak

Background and Purpose: Neovascular retinal pathology is steel uncertain. Thus, there is great need to investigate new modeling, diagnostic and treatment technologies. Brinzolamide induces a metabolic acidosis via an alternative biochemical mechanism (bicarbonate loss). In the present study the influence of brinzolamide-induced acidosis on preretinal neovascularization in neonatal rat was investigated.

Materials and Methods. In our study we used newborn Wistar rats
raised in two litters. Newborn rats (n=17) were randomized to either intraperitoneal brinzolamide in dose 200 mg/kg (n=9) or saline (n=8), twice daily from days 2 to 7. After 5 days of recovery all rats were euthanized. Six paraffin-embedded sections prepared in a standard manner and stained with hematoxylin and eosin. To investigate the damage, we evaluated the number of cells in the ganglion cell layer (GCL) and the thicknesses of the inner plexiform layer (IPL), inner nuclear layer (INL), outer plexiform layer (OPL), and outer nuclear layer (ONL). The cells in GCL and the thicknesses of the IPL, INL, and ONL at a distance between 375 and 625 μm from the optic disc were measured.

Results: Preretinal neovascularization was found in 71% retinas, from
brinzolamide-injected rats. Cataract formation was detected in 100% eyes after brinzolamide injections. All retinas from saline group were negative for neither neovascularization nor cataract. Brinzolamide rat model retinas showed decrease in the thicknesses of the retinal layers.
Conclusion: In the present study, we report that brinzolamide (a drug
causing metabolic acidosis primarily by bicarbonate loss) is associated with preretinal neovascularization in the neonatal rat. So this method can be suitable model for other neovascular pathologies of the eye.

Ključne riječi

Hrčak ID:

125479

URI

https://hrcak.srce.hr/125479

Datum izdavanja:

31.3.2014.

Posjeta: 1.191 *