Izvorni znanstveni članak
https://doi.org/10.3325/cmj.2014.55.45
Increased survival after irradiation followed by regeneration of bone marrow stromal cells with a novel thiolbased radioprotector
Ivana Okić-Djordjević
; Institute for Medical Research University of Belgrade, Belgrade, Serbia
Drenka Trivanović
; Institute for Medical Research University of Belgrade, Belgrade, Serbia
Miloš Jovanović
; Faculty of Biology, University of Belgrade, Belgrade, Serbia
Marija Ignjatović
; Vinča Institute of Nuclear Sciences, University of Belgrade, Belgrade, Serbia
Bojana Šećerov
; Vinča Institute of Nuclear Sciences, University of Belgrade, Belgrade, Serbia
Miloš Mojović
; Faculty of Physical Chemistry, University of Belgrade, Belgrade, Serbia
Diana Bugarski
; Institute for Medical Research University of Belgrade, Belgrade, Serbia
Goran Bačić
; Faculty of Physical Chemistry, University of Belgrade, Belgrade, Serbia
Pavle R. Andjus
orcid.org/0000-0002-8468-8513
; Faculty of Biology, University of Belgrade, Belgrade, Serbia
Sažetak
Aim To investigate the survival of laboratory rats after irradiation
and to study the cellularity of their bone marrow
and the multipotential mesenchymal stem cells (BMMSCs)
in groups treated with or without a new thiol-based
radioprotector (GM2011)
Methods Animals were irradiated by a Cobalt gamma
source at 6.7 Gy. Treated animals were given i.p. GM2011
30 minutes before and 3 and 7 hours after irradiation. Controls
consisted of sham irradiated animals without treatment
and animals treated without irradiation. After 30 days
post-irradiation, animals were sacrificed and bone marrow
cells were prepared from isolated femurs. A colony forming
unit-fibroblast (CFU-F) assay was performed to obtain the
number of BM-MSCs.
Results In the treated group, 87% of animals survived,
compared to only 30% in the non-treated irradiated group.
Irradiation induced significant changes in the bone marrow
of the treated rats (total bone marrow cellularity was
reduced by ~ 60% – from 63 to 28 cells ×106/femur and the
frequency of the CFU-F per femur by ~ 70% – from 357 to
97), however GL2011 almost completely prevented the
suppressive effect observed on day 30 post-irradiation (71
cells ×106/femur and 230 CFU-F/femur).
Conclusion Although the irradiation dosage was relatively
high, GL2011 acted as a very effective new radioprotector.
The recovery of the BN-MSCs and their counts support
the effectiveness of the studied radioprotector.
Ključne riječi
Hrčak ID:
127273
URI
Datum izdavanja:
15.2.2014.
Posjeta: 1.319 *