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Langmuir Aggregation of Dahliaviolett on DNA

Hong-Wen Gao ; College of Chemical Engineering, Zhejiang University of Technology, Hangzhou-310014, P. R. China

Puni tekst: engleski pdf 118 Kb

str. 683-692

preuzimanja: 246



Formation of double electrostatic films in DNA causes Langmuir aggregation of small ions and molecules only in a monolayer. We studied the interaction of dahliaviolett (DLV) with DNA at pH = 7.24 by a spectral correction technique. Results showed that the ad-sorption ratio of DLV to DNA-P was 1 : 4, the adsorption constant of the DLV-DNA aggregate was 4.44 x 104 and its molar absorption coefficient was 1.02 x 105 dm3 mol-1 cm-1 at 615 nm. This aggregation was applied to the quantitative determination of DNA in samples.

Ključne riječi

DNA, dahliaviolett, double electrostatic films, Langmuir aggregation, spectral correction technique

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