Izvorni znanstveni članak
https://doi.org/10.15255/CABEQ.2013.1885
Xylanase Production by Penicillium citrinum in Laboratory-scale Stirred Tank Reactor
G. Ghoshal
; Dr. S. S. Bhatnagar University Institute of Chemical Engineering and Technology, Panjab University, Chandigarh – 160014, India
U. C. Banerjee
; Department of Pharmaceutical Technology, National Institute of Pharmaceutical Education and Research, SAS Nagar-160062, Panjab, India
U. S. Shivhare
; Dr. S. S. Bhatnagar University Institute of Chemical Engineering and Technology, Panjab University, Chandigarh – 160014, India
Sažetak
Xylanase constitutes an important class of hydrolases, and is used in numerous industrial applications. The aim of the present work was to study the production of xylanase from Penicillium citrinum MTCC 9620 in a 5 L stirred tank bioreactor. Effect of various process parameters; pH, temperature, aeration, agitation rates, substrate concentration, and, dissolved oxygen (DO) concentration on xylanase production were studied. Combination of all the optimized parameters resulted in 2.5 times higher enzyme activity as compared to the shake flask fermentation after 96 h. Effect of varying agitation and aeration on the volumetric mass transfer coefficient (KLa) was determined. It revealed that KLa is influenced by both aeration and agitation. Growth kinetics of P. citrinum MTCC 9620 in bioreactor was studied using Monod, Moser, Contois and Edward equation. Based on R2, SE and pattern of residuals, the microbial growth kinetics of P. citrinum MTCC 9620 was effectively represented by Moser equation.
Ključne riječi
xylanase; Penicillium citrinum; sugarcane bagasse; volumetric mass transfer coefficient; kinetics models
Hrčak ID:
128046
URI
Datum izdavanja:
10.10.2014.
Posjeta: 1.254 *