Izvorni znanstveni članak
https://doi.org/10.3325/cmj.2014.55.239
Single acute stress-induced progesterone and ovariectomy alter cardiomyocyte contractile function in female rats
Judit Kalász
; University of Debrecen, Faculty of Medicine, Institute of Cardiology, Division of Clinical Physiology, Debrecen, Hungary
Enikő Pásztor Tóth
; University of Debrecen, Faculty of Medicine, Institute of Cardiology, Division of Clinical Physiology, Debrecen, Hungary
Beáta Bódi
; University of Debrecen, Faculty of Medicine, Institute of Cardiology, Division of Clinical Physiology, Debrecen, Hungary
Miklós Fagyas
; University of Debrecen, Faculty of Medicine, Institute of Cardiology, Division of Clinical Physiology, Debrecen, Hungary
Attila Tóth
; University of Debrecen, Faculty of Medicine, Institute of Cardiology, Division of Clinical Physiology, Debrecen, Hungary
Bhattoa Harjit Pal
; University of Debrecen, Faculty of Medicine, Department of Laboratory Medicine, Debrecen, Hungary
Sándor G. Vári
; Cedars-Sinai Medical Center, International Research and Innovation Management Program, Los Angeles, CA, USA
Marta Balog
; J. J. Strossmayer University of Osijek, School of Medicine Department of Medical Biology Osijek, Croatia
Marija Heffer
; J. J. Strossmayer University of Osijek, School of Medicine Department of Medical Biology Osijek, Croatia
Zoltán Papp
; University of Debrecen, Faculty of Medicine, Institute of Cardiology, Division of Clinical Physiology, Debrecen, Hungary
Attila Borbély
; University of Debrecen, Faculty of Medicine, Institute of Cardiology, Division of Clinical Physiology, Debrecen, Hungary
Sažetak
Aim To assess how ovarian-derived sex hormones (in particular
progesterone) modify the effects of single acute
stress on the mechanical and biochemical properties of
left ventricular cardiomyocytes in the rat.
Methods Non-ovariectomized (control, n = 8) and ovariectomized
(OVX, n = 8) female rats were kept under normal
conditions or were exposed to stress (control-S, n = 8
and OVX-S, n = 8). Serum progesterone levels were measured
using a chemiluminescent immunoassay. Left ventricular
myocardial samples were used for isometric force
measurements and protein analysis. Ca2+-dependent active
force (Factive), Ca2+-independent passive force (Fpassive),
and Ca2+-sensitivity of force production were determined
in single, mechanically isolated, permeabilized cardiomyocytes.
Stress- and ovariectomy-induced alterations in myofilament
proteins (myosin-binding protein C [MyBP-C], troponin
I [TnI], and titin) were analyzed by sodium dodecyl
sulfate gel electrophoresis using protein and phosphoprotein
stainings. Results Serum progesterone levels were significantly increased
in stressed rats (control-S, 35.6 ± 4.8 ng/mL and
OVX-S, 21.9 ± 4.0 ng/mL) compared to control (10 ± 2.9
ng/mL) and OVX (2.8 ± 0.5 ng/mL) groups. Factive was higher
in the OVX groups (OVX, 25.9 ± 3.4 kN/m2 and OVX-S,
26.3 ± 3.0 kN/m2) than in control groups (control, 16.4 ± 1.2
kN/m2 and control-S, 14.4 ± 0.9 kN/m2). Regarding the
potential molecular mechanisms, Factive correlated with
MyBP-C phosphorylation, while myofilament Ca2+-sensitivity
inversely correlated with serum progesterone levels
when the mean values were plotted for all animal groups.
Fpassive was unaffected by any treatment.
Conclusion Stress increases ovary-independent synthesis
and release of progesterone, which may regulate Ca2+-sensitivity
of force production in left ventricular cardiomyocytes.
Stress and female hormones differently alter Ca2+-
dependent cardiomyocyte contractile force production,
which may have pathophysiological importance during
stress conditions affecting postmenopausal women.
Ključne riječi
Hrčak ID:
129839
URI
Datum izdavanja:
15.6.2014.
Posjeta: 1.586 *