Izvorni znanstveni članak

Effects of lead on the development of Drosophila melanogaster

SABEREH SAFAEE orcid.org/0000-0001-5250-898X ; Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran
MASOUD FEREIDONI
NASER MAHDAVI-SHAHRI
FARHANG HADDAD

Sažetak

Background and Purpose: Lead as a heavy metal and environmental
pollutant causes sperm abnormalities, organogenesis, morphogenesis disorders and miscarriage. There are some similarities between mammals and Drosophila melanogaster. The present study is to investigate the lead-ion effects on some developmental aspects of Drosophila as a model.

Materials and Methods: Five pairs of three-day flies were added to the
culture containing different concentrations of lead-ion to mate and lay eggs. Transformation rate of larvae to pupa, pupa to adult, the required time for insect development and morphometric changes as well as eggs hatching rate for insects that developed in culture containing lead-ion were studied.

Results: Culture-medium lead-ion (20-300 mg/L) increased larvae and
pupae periods, but decreased the conversion rate of larvae to pupa, pupa to adult and eggs hatching. It also decreased the growth rate of larvae length/ width, pupa length/width and adult length. The results show that eggs length/width did not change.

Conclusion: Factors such as the lead-ion interference with enzymes
performances were involved in metamorphosis, reducing the mitochondrial cristae and ATP synthesis. In addition, the negative effect of lead on the production of growth hormones, metabolic enzymes and genes expression are the suggesting aspects for future study.

Ključne riječi

Egg Hatching; Embryo; Larvae; Lead-ion; Pupa; Teratogenicity

Hrčak ID:

135138

URI

https://hrcak.srce.hr/135138

Datum izdavanja:

30.9.2014.

Posjeta: 3.449 *