Izvorni znanstveni članak
https://doi.org/10.3325/cmj.2015.56.78
Protective effect of resveratrol against caspase 3 activation in primary mouse fibroblasts
Zsófia Ulakcsai
; Department of Pharmacodynamics,Semmelweis University, Budapest,Hungary
Fruzsina Bagaméry
; Department of Pharmacodynamics,Semmelweis University, Budapest,Hungary
István Vincze
; Department of Pharmacodynamics,Semmelweis University, Budapest,Hungary
Éva Szökő
; Department of Pharmacodynamics,Semmelweis University, Budapest,Hungary
Tamás Tábi
; Department of Pharmacodynamics,Semmelweis University, Budapest,Hungary
Sažetak
Aim To study the effect of resveratrol on survival and caspase
3 activation in non-transformed cells after serum deprivation.
Methods Apoptosis was induced by serum deprivation
in primary mouse embryonic fibroblasts. Caspase 3 activation
and lactate dehydrogenase release were assayed as
cell viability measure by using their fluorogenic substrates.
The involvement of PI3K, ERK, JNK, p38, and SIRT1 signaling
pathways was also examined.
Results Serum deprivation of primary fibroblasts induced
significant activation of caspase 3 within 3 hours and reduced
cell viability after 24 hours. Resveratrol dose-dependently
prevented caspase activation and improved cell viability
with 50% inhibitory concentration (IC50) = 66.3 ± 13.81
μM. It also reduced the already up-regulated caspase 3 activity
when it was added to the cell culture medium after
3 hour serum deprivation, suggesting its rescue effect.
Among the major signaling pathways, p38 kinase was critical
for the protective effect of resveratrol which was abolished
completely in the presence of p38 inhibitor.
Conclusion Resveratrol showed protective effect against
cell death in a rather high dose. Involvement of p38 kinase
in this effect suggests the role of mild stress in its cytoprotective
action. Furthermore due to its rescue effect,
resveratrol may be used not only for prevention, but also
treatment of age-related degenerative diseases, but in the
higher dose than consumed in conventional diet.
Ključne riječi
Hrčak ID:
139298
URI
Datum izdavanja:
15.4.2015.
Posjeta: 1.019 *