Original scientific paper

Effects of herbaceous plant preparation of Cistanche on in vitro cytokine expression by rat osteoblasts.

Caihong Li ; College of Veterinary Medicine, China Agricultural University, Beijing, PR China
Qing Li ; College of Veterinary Medicine, China Agricultural University, Beijing, PR China
Zhongjie Liu ; College of Veterinary Medicine, China Agricultural University, Beijing, PR China
Xiaoxu Xing ; College of Veterinary Medicine, China Agricultural University, Beijing, PR China
Dan Tian ; College of Veterinary Medicine, China Agricultural University, Beijing, PR China
Zoran Vrbanac ; Department of Radiology, Ultrasound Diagnostics and Physical Therapy, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Ozren Smolec ; Clinic for Surgery, Orthopedy and Ophtalmology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Damir Stanin ; Department of Radiology, Ultrasound Diagnostics and Physical Therapy, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Josip Kos ; Clinic for Surgery, Orthopedy and Ophtalmology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia

Abstract

Osteoblasts (OB) play an important role in bone remodeling through the release of cytokines such as osteoprotegerin (OPG), a receptor activator of nuclear factor kappa-B ligand (RANKL) and osteopontin (OPN). The objective of our study was to evaluate the effects of Chinese herb extracts of Cistanche (Cistanche deserticola) on the in vitro expression of these cytokines in rat OB cell culture. The primary OBs were isolated from the rat skull by trypsin and collagenase digestion. The cellular phenotype was determined by the Swiss dyeing method, alkaline phosphatase staining, Von Kossa staining, and Alizarin red staining. The gene expression of OPG, RANKL and OPN were analyzed by quantitative real-time PCR. All treatments with herbaceous plant extracts of Cistanche increased the gene expression of OPG and RANKL at 72 hours of culturing, especially at concentrations of 5×10-2 mg/mL, which were significantly different compared to the control cultures. Treatment significantly reduced the expression of the OPG/RANKL ratio at 48 hours and the expression of OPN mRNA at 72 hours of culturing. The in vitro exposure of OB to the plant preparation of Cistanche promoted the gene expression of OPG and RANKL and reduced the gene expression of OPG/RANKL and OPN, and thus, could maintain the balance of bone formation and resorption during bone metabolism.

Keywords

Cistanche; osteoblasts; osteoprotegerin; RANKL; osteopontin; rat

Hrčak ID:

141045

URI

https://hrcak.srce.hr/141045

Publication date:

26.6.2015.

Article data in other languages: croatian

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