Veterinarski arhiv, Vol. 85 No. 3, 2015.
Recenzija, Prikaz slučaja
Chlamydophila abortus infection in a flock of goats in Bosnia and Herzegovina.
Lejla Krkalić
; Department of State and Forensic Veterinary Medicine, Veterinary Faculty Sarajevo, University of Sarajevo, Sarajevo, Bosnia and Herzegovina
Edin Šatrović
; Department of State and Forensic Veterinary Medicine, Veterinary Faculty Sarajevo, University of Sarajevo, Sarajevo, Bosnia and Herzegovina
Teufik Goletić
; Department of Avian Diseases and Management, Veterinary Faculty Sarajevo, University of Sarajevo, Sarajevo, Bosnia and Herzegovina
Petar Džaja
; Department of State and Forensic Veterinary Medicine, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Krešimir Severin
; Department of State and Forensic Veterinary Medicine, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Sažetak
The aim of this research was to determine the presence of Chlamydophila abortus (C. abortus) infection in one flock of goats with a previously recorded history of reproductive failures (abortion, stillbirths, weak born kids) and long-term poor reproductive performances. The affected flock was from the southern region of Bosnia and Herzegovina (B&H) and consisted of 48 goats kept in semi-intensive conditions. Blood samples and vaginal swabs were collected twice during 2012 and 2013 and the sample size was estimated at a 95 % confidence level, with predicted prevalence of 20 %, using the recommendations for determining the required sample size necessary to detect the presence of disease in a flock. A representative sample from this flock was taken by simple random sampling. In the total of 12 blood sera that were tested for the specific antibodies against C. abortus, with the use of enzyme-linked immunosorbent assay (CHEKIT® Chlamydophila abortus Antibody Test Kit), the results showed that 11 (91.7 %) sera were positive for C. abortus antibodies. Vaginal swabs from all animals were analysed by a modified Chlamydiaceae-specific rtPCR test, targeting the 23S rRNA gene, to determine the presence of known Chlamydiaceae, and three (25 %) samples were positive. These positive samples were subsequently tested with a test targeting the ompA gene region (ompA-rtPCR) specific for Chlamydophila abortus. All three samples were also positive using this test.
Ključne riječi
Chlamydophila abortus; goats; flock; Bosnia and Herzegovina
Hrčak ID:
141047
URI
Datum izdavanja:
26.6.2015.
Posjeta: 1.741 *