Prethodno priopćenje

Eutypella parasitica R.W. Davidson & R.C. Lorenz SEVEN YEARS AFTER THE FIRST FINDINGS IN CROATIA

Sanja Novak Agbaba orcid.org/0000-0002-7077-2204 ; Hrvatski šumarski institut, Jastrebarsko
Nevenka Ćelepirović orcid.org/0000-0002-4183-5309 ; Hrvatski šumarski institut, Jastrebarsko
Monika Karija Vlahović orcid.org/0000-0003-0001-4624 ; DNA laboratorij Zavoda za sudsku medicinu i kriminalistiku, Medicinski fakultet Sveučilišta u Zagrebu
Boris Liović orcid.org/0000-0002-0824-1648 ; Hrvatski šumarski institut, Jastrebarsko

Sažetak

Fungus Eutypella parasitica is cause of bark canker on on maple trees. The disease reduces the economic and aesthetic value of maple. In Europe, the Eutypella canker was first recorded in Slovenia in 2005. From 2005 to 2008 the disease was listed on the EPPO Alert List because of the danger of contagion. In Croatia, Eutypella canker was recorded for the first time in 2007 on A. campestre near the border with Slovenia. The program Special monitoring of fungus E. parasitica was carried out since 2011 in Croatia. The aim of this study was to determine the status of infection on maples seven years after it was recorded for the first time, spread of the disease and infection intensity in Croatia. The second objective was to confirm the type of fungus using phytopathological and molecular analysis and store the DNA sequence of ITS2 region in GenBank (NCBI). The forest stands that represent maple trees in the area of 8 counties and 17 localities were examined in the period from 2011 to 2014. From a total of 2209 examined trees, only 2 trees of A. pseudoplatanus,and A. campestre, in the vicinity of settlement Hum na Sutli on the Slovenian border, were infected by the fungus, representing
only 0.09 % of infected trees. Eutypella canker was found with very low intensity and the disease has not spread to other sites. The samples of cankerous formations were submitted to laboratory analysis, morphological analysis, and DNA analysis to confirm the presence of fungi E. parasitica. Fragments of DNA were sequenced and compared with gene in databases GenBank (NCBI), where it was confirmed that it was a 100 % identity with the E. parasitica. ITS2 region fungus E.parasitica established as sycamore and chubs was sequenced and deposited in gene bank (GenBank, NCBI) under accession numbers KP868619 and KP868620. Fungus E. parasitica was recorded for the first time in Croatia on A. pseudoplatanus in 2013, and its presence was confirmed on A. campestre in 2014. Type fungus E. parasitica was confirmed by morphological and molecular DNA detection. Bark canker on maples has not been significantly expanded during the last seven years in Croatia.

Ključne riječi

<i>Eutypella parasitica</i>; <i>Eutypella canker</i>; <i>Acer pseudoplatanus</i>; <i>Acer campestre</i>; molecular diagnostic; ITS2

Hrčak ID:

141900

URI

https://hrcak.srce.hr/141900

Datum izdavanja:

30.6.2015.

Podaci na drugim jezicima: hrvatski

Posjeta: 1.699 *