Izvorni znanstveni članak
https://doi.org/10.15255/CABEQ.2014.2058
Study on the Production and Re-use of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) and Extracellular Polysaccharide by the Archaeon Haloferax mediterranei Strain DSM 1411
M. Koller
orcid.org/0000-0002-9251-1822
; a)University of Graz, Institute of Chemistry, Division of Physical and Theoretical Chemistry, Research group Interfaces, NAWI Graz, Heinrichstrasse 28, A-8010 Graz, Austria;b)ARENA – Association for Resource Efficient and Sustainable Technologies; Inffeld
E. Chiellini
; Department of Chemistry & Industrial Chemistry, University of Pisa, Via Risorgimento, 35 – 56126 – Pisa, Italy
G. Braunegg
; ARENA – Association for Resource Efficient and Sustainable Technologies; Inffeldgasse 23, 8010 Graz, Austria
Sažetak
The halobacterium Haloferax mediterranei was used to study the production of two types of biopolymers: The biopolyester poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) was accumulated as intracellular granules, whereas an extracelluar polysaccharide was excreted in parallel to biopolyester synthesis. After production, microbial re-use and degradation of these polymers under different conditions were investigated to assess the requirements for handling the product-rich fermentation broth prior to the downstream processing for product recovery. Degradation kinetics of the polymers and the impact of different storage conditions on molar mass of PHBV were studied. It turned out that the biotechnological fermentation process can be run without any sterility precautions. No major product losses were observed without pasteurization of fermentation broth after the stop of fermentation. In addition, neither PHBV nor EPS are re-utilized by the cells for biomass formation even if the culture is maintained under conditions of carbon starvation for an extended time.
Ključne riječi
Archaea; copolyester; degradation; extracellular polysaccharide (EPS); Haloferax mediterranei; poly(hydroxyalkanoate) (PHA); poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV)
Hrčak ID:
141904
URI
Datum izdavanja:
18.7.2015.
Posjeta: 1.637 *