Acta Pharmaceutica, Vol. 65 No. 4, 2015.
Izvorni znanstveni članak
https://doi.org/10.1515/acph-2015-0032
Nitrones: not only extraordinary spin traps, but also good nitric oxide sources in vivo
MIRCEA DUMITRU CROITORU
; Faculty of Pharmacy, University of Medicine and Pharmacy of Tîrgu Mureş, Tîrgu Mureş 540138, Romania
HERMINA IULIA PETKES
; Faculty of Chemistry and Chemical Engineering, Babes-Bolyai University, Cluj-Napoca 400028, Romania
IBOLYA FÜLÖP
; Faculty of Pharmacy, University of Medicine and Pharmacy of Tîrgu Mureş, Tîrgu Mureş 540138, Romania
REMUS COTÂRLAN
; Faculty of Pharmacy, University of Medicine and Pharmacy of Tîrgu Mureş, Tîrgu Mureş 540138, Romania
OANA ELENA ŞERBAN
; Faculty of Chemistry and Chemical Engineering, Babes-Bolyai University, Cluj-Napoca 400028, Romania
TITICA MARIA DOGARU
; Faculty of Pharmacy, University of Medicine and Pharmacy of Tîrgu Mureş, Tîrgu Mureş 540138, Romania
ŞERBAN ANDREI GÂZ FLOREA
; Faculty of Pharmacy, University of Medicine and Pharmacy of Tîrgu Mureş, Tîrgu Mureş 540138, Romania
BÉLA TŐKÉS
; Faculty of Pharmacy, University of Medicine and Pharmacy of Tîrgu Mureş, Tîrgu Mureş 540138, Romania
CORNELIA MAJDIK
; Faculty of Chemistry and Chemical Engineering, Babes-Bolyai University, Cluj-Napoca 400028, Romania
Sažetak
Free radicals are involved in the development of reperfusion injuries. Using a spin trap, the intensity of such lesions can be reduced. Nitrones (effective in vivo spin traps) were tried in this work as in vivo nitric oxide donors. Nitrite and nitrate concentration values (rabbit blood) were used as biomarkers of nitric oxide production. Most nitrones did not increase plasma concentrations of nitrite and nitrate; on the contrary, reduced plasma concentrations of these indicators were noted. However, glyoxal isopropyldinitrone, in a dose of 50 mg kg–1, was highly effective in increasing nitric oxide production. In the same time, nitrones do not react with hepatic homogenates, proving that the release of nitric oxide takes place in the tissues and is not related to hepatic metabolism. Before using nitrones in vivo, they were tested in vitro for the ability to release nitric oxide following a reaction with the hydroxyl radical.
Ključne riječi
nitric oxide; nitrite; nitrate; nitrones; nitric oxide donor
Hrčak ID:
142804
URI
Datum izdavanja:
31.12.2015.
Posjeta: 1.757 *