Izvorni znanstveni članak
https://doi.org/10.3325/cmj.2015.56.239
Evaluation of blood, buccal swabs, and hair follicles for DNA profiling technique using STR markers
Garima Chaudhary
; Department of Forensic Medicine All India Institute of Medical Sciences, New Delhi, India
TD Dogra
; SGT Group of Institutions, Budhera Gurgaon, Haryana, India
Anupuma Raina
orcid.org/0000-0002-5988-079X
; Department of Forensic Medicine All India Institute of Medical Sciences, New Delhi, India
Sažetak
Aim To study the short tandem repeat (STR) pattern of
DNA from the blood, buccal swabs, and hair follicles of the
recipients of allogenic hematopoietic stem cell transplantation
to examine whether these tissues contain donor derived
cells.
Methods The study enrolled 25 patients who sustained
engraftment. Peripheral blood, buccal swabs, and hair follicles
were collected on days 21-30, 90, and 180 after transplantation
and the chimeric status of the recipients was
evaluated.
Results Donor derived cells existed in the blood and buccal
swabs, but not in hair follicles, which can be used to obtain
the pre-transplant sample of the recipient after transplant.
Conclusion Peripheral blood and buccal swab do not
serve as a reliable source of recipient’s origin for DNA analysis
of individuals who underwent allogenic hematopoietic
stem cell transplantation at least within 6 months after
transplant.
Ključne riječi
Hrčak ID:
146558
URI
Datum izdavanja:
15.6.2015.
Posjeta: 1.553 *