Izvorni znanstveni članak
https://doi.org/10.3325/cmj.2015.56.431
Effects of d- and l-limonene on the pregnant rat myometrium in vitro
Judit Hajagos-Tóth
; Department of Pharmacodynamics and Biopharmacy, Faculty of Pharmacy, University of Szeged,Szeged, Hungary
Ágnes Hódi
; Department of Pharmacodynamics and Biopharmacy, Faculty of Pharmacy, University of Szeged,Szeged, Hungary
Adrienn B. Seres
; Department of Pharmacodynamics and Biopharmacy, Faculty of Pharmacy, University of Szeged,Szeged, Hungary
Róbert Gáspár
; Department of Pharmacodynamics and Biopharmacy, Faculty of Pharmacy, University of Szeged,Szeged, Hungary
Sažetak
Aim To study the effects of d- and l-limonene on pregnant
rat myometrial contractility in vitro, and investigate
how these effects are modified by other agents. D- and llimonene
(10−13-10−8 M) caused myometrial contraction in
a dose-dependent manner.
Methods Contractions of uterine rings from 22-day-pregnant
rats were measured in an organ bath in the presence
of d- or l-limonene (10−13-10−8 M) and nifedipine (10−8 M),
tetraethyl-ammonium (10−3 M), theophylline (10−5 M), or
paxilline (10−5 M). Uterine cyclic adenosine monophosphate
(cAMP) level was detected by enzyme immunoassay.
Oxidative damage was induced by methylglyoxal
(3 × 10−2 M) and the alteration was measured via noradrenaline
(1 × 10−9 to 3 × 10−5 M) -induced contractions.
Results Pre-treatment with nifedipine (10−8 M), tetraethylammonium
(10−3 M), and theophylline (10−5 M) attenuated
the contracting effect of d- and l-limonene, while in
the presence of paxilline (10−5 M) d- and l-limonene were
ineffective. The two enantiomers decreased the myometrial
cAMP level, but after paxilline pretreatment the cAMP
level was not altered compared with the control value. Additionally,
l-limonene (10−6 M) diminished consequences of
oxidative damage caused by methylglyoxal (3 × 10−2 M) on
contractility, whereas d-limonene was ineffective.
Conclusion Our findings suggest that l-limonene has an
antioxidant effect and that both d-and l-limonene cause
myometrial contraction through activation of the A2A receptor
and opening of the voltage-gated Ca2+ channel.
It is possible that limonene-containing products increase
the pregnant uterus contractility and their use should be
avoided during pregnancy.
Ključne riječi
Hrčak ID:
151735
URI
Datum izdavanja:
15.10.2015.
Posjeta: 1.485 *