Izvorni znanstveni članak
https://doi.org/10.3325/cmj.2015.56.482
Identification and characterization of the highly polymorphic locus D14S739 in the Han Chinese population
Chengchen Shao
; Department of Forensic Medicine Shanghai Medical College of Fudan University, Shanghai, China
Yaqi Zhang
; Department of Forensic Medicine Shanghai Medical College of Fudan University, Shanghai, China
Yueqin Zhou
; Department of Forensic Medicine Shanghai Medical College of Fudan University, Shanghai, China
Wei Zhu
; Department of Forensic Medicine Shanghai Medical College of Fudan University, Shanghai, China
Hongmei Xu
; Department of Forensic Medicine Shanghai Medical College of Fudan University, Shanghai, China
Zhiping Liu
; Department of Forensic Medicine Shanghai Medical College of Fudan University, Shanghai, China
Qiqun Tang
; Department of Biochemistry and Molecular Biology, Shanghai Medical College of Fudan University, Shanghai, China
Yiwen Shen
; Department of Forensic Medicine Shanghai Medical College of Fudan University, Shanghai, China
Jianhui Xie
; Department of Forensic Medicine Shanghai Medical College of Fudan University, Shanghai, China
Sažetak
Aim To systemically select and evaluate short tandem repeats
(STRs) on the chromosome 14 and obtain new STR
loci as expanded genotyping markers for forensic application.
Methods STRs on the chromosome 14 were filtered from
Tandem Repeats Database and further selected based on
their positions on the chromosome, repeat patterns of the
core sequences, sequence homology of the flanking regions,
and suitability of flanking regions in primer design.
The STR locus with the highest heterozygosity and polymorphism
information content (PIC) was selected for further
analysis of genetic polymorphism, forensic parameters,
and the core sequence.
Results Among 26 STR loci selected as candidates,
D14S739 had the highest heterozygosity (0.8691) and PIC
(0.8432), and showed no deviation from the Hardy-Weinberg
equilibrium. 14 alleles were observed, ranging in size
from 21 to 34 tetranucleotide units in the core region of
(GATA)9-18 (GACA)7-12 GACG (GACA)2 GATA. Paternity testing
showed no mutations.
Conclusion D14S739 is a highly informative STR locus and
could be a suitable genetic marker for forensic applications
in the Han Chinese population
Ključne riječi
Hrčak ID:
153073
URI
Datum izdavanja:
15.10.2015.
Posjeta: 1.053 *