Veterinarski arhiv, Vol. 86 No. 2, 2016.
Izvorni znanstveni članak
Antimicrobial susceptibility of milk bacteria from healthy and drugtreated cow udder.
Nevijo Zdolec
orcid.org/0000-0001-7413-8115
; Department of Hygiene, Technology and Food Safety, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Vesna Dobranić
; Department of Hygiene, Technology and Food Safety, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Ivan Butković
; students, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Ana Koturić
; students, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Ivana Filipović
; Department of Hygiene, Technology and Food Safety, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Vinko Medvid
; Veterinary Station d.o.o., Zaprešić, Croatia
Sažetak
The aim of this study was to evaluate the antimicrobial susceptibility of milk microbiota, considering udder health status and drug treatment history. Composite milk samples were taken aseptically from healthy cows without any signs of mastitis (n = 17) and drug-treated cows with cured mastitis (n = 19). Antimicrobial susceptibility testing was performed for 56 enterococci, 30 Escherichia coli, 24 enterobacteria and 94 staphylococci. Depending on the bacterial group or species, the following antibiotic disks were used: ampicillin, rifampin, chloramphenicol, linezolid, tetracycline, erythromycin, nitrofurantoin, vancomycin, penicillin, trimethoprim, cefoperazone, kanamycin, trimethoprim/sulfamethoxazole, nalidixic acid, ciprofloxacin, gentamicin, teicoplanin, sulfonamides, levofloxacin, clindamycin and amoxicillin/clavulanic acid. The occurrence of multiresistant E. coli and staphylococci was significantly higher (P<0.05) in the milk of drug-treated cows. The percentage ratio (drug-treated: healthy udders) of multiresistant E. coli was 56.3:0, enterobacteria 57.14:30 and staphylococci 56.5: 4.8. There was no significant difference (P>0.05) between the prevalence of multiresistant enterococci in the milk of healthy cows and cows with cured mastitis (87.2:73.7). The high prevalence of resistance in enterococci isolated from milk samples of healthy cows could be the result of animal cohabitation and cross-contamination.
Ključne riječi
antimicrobial resistance; milk; enterococci; udder health
Hrčak ID:
156704
URI
Datum izdavanja:
22.4.2016.
Posjeta: 1.902 *