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Brucellosis in Turopolje pig breeding in the 2008-2011 period: an overview of laboratory diagnostics and eradication system.

Silvio Špičić orcid.org/0000-0001-8317-5831 ; Croatian Veterinary Institute, Zagreb, Croatia
Miroslav Benić ; Croatian Veterinary Institute, Zagreb, Croatia
Maja Zdelar-Tuk ; Croatian Veterinary Institute, Zagreb, Croatia
Sanja Duvnjak ; Croatian Veterinary Institute, Zagreb, Croatia
Aneta Karakaš ; Ministry of Agriculture, Directorate of Veterinary Services and Food Safety, Zagreb, Croatia
Tomislav Kiš ; Ministry of Agriculture, Directorate of Veterinary Services and Food Safety, Zagreb, Croatia
Ivica Sučec ; Ministry of Agriculture, Directorate of Veterinary Services and Food Safety, Zagreb, Croatia
Željko Cvetnić ; Croatian Veterinary Institute, Zagreb, Croatia

Sažetak

In the period 2008-2011, a program of diagnostics and control of swine brucellosis was conducted in the remaining two farms of the Turopolje pig breed, including a total of 729 samples of porcine blood. Serological diagnostics were carried out by means of the Rose Bengal test (RBT), complement fixation test (CFT) and enzyme-linked immunosorbent assay (ELISA). Agreement between serological methods was determined by the Kappa test. Almost perfect agreement was found between the results obtained by the CFT and RBT methods (0.764-0.936), while the agreement between the results obtained by RBT and CFT in comparison with the ELISA test results was substantial (0.547-0.767; 0.647-0.843). In addition, in 2009 and 2011, samples from 50 pigs were subjected to bacteriological tests, and a total of 15 isolates were obtained. Bacteriology was conducted with various time delays from the determination of positive serological reactions, and the method efficacy was determined considering the duration of the infection in pigs. The average efficacy of the isolation of Brucella (B.) suis ranged between 10-43.3 % (P = 0.01) and was significantly higher in acute brucellosis. Isolation was successful for all age categories, regardless of their sexual maturity. Molecular identification confirmed B. suis bv. 2 for the first time in this breed. The extensive breeding of Turopolje pigs and daily contact with live reservoirs of the disease agents are the reasons why the disease cannot be completely eradicated according to the test-and-remove principle. A condition for the successful control and eradication of brucellosis in a Turopolje pig herd is to ensure the conditions for individual and permanent supervision of each breeding pig.

Ključne riječi

swine brucellosis; Brucella suis; Turopolje pig; Croatia

Hrčak ID:

156841

URI

https://hrcak.srce.hr/156841

Datum izdavanja:

22.4.2016.

Podaci na drugim jezicima: hrvatski

Posjeta: 1.096 *