Izvorni znanstveni članak
ELISA-like Analysis of Cisplatinated DNA Using Magnetic Separation
Krystina Smerkova
; Department of Chemistry and Biochemistry, Laboratory of Metallomics and Nanotechnology, Mendel University in Brno, Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic
Marcela Vlcnovska
; Department of Chemistry and Biochemistry, Laboratory of Metallomics and Nanotechnology, Mendel University in Brno, Brno, Czech Republic
Simona Dostalova
; Department of Chemistry and Biochemistry, Laboratory of Metallomics and Nanotechnology, Mendel University in Brno, Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic
Vedran Milosavljevic
; Department of Chemistry and Biochemistry, Laboratory of Metallomics and Nanotechnology, Mendel University in Brno, Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic
Pavel Kopel
; Department of Chemistry and Biochemistry, Laboratory of Metallomics and Nanotechnology, Mendel University in Brno, Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic
Tomas Vaculovic
; Department of Chemistry, Faculty of Science, Masaryk University, Brno, Czech Republic
Sona Krizkova
; Department of Chemistry and Biochemistry, Laboratory of Metallomics and Nanotechnology, Mendel University in Brno, Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic
Marketa Vaculovicova
; Department of Chemistry and Biochemistry, Laboratory of Metallomics and Nanotechnology, Mendel University in Brno, Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic
Vojtech Adam
; Department of Chemistry and Biochemistry, Laboratory of Metallomics and Nanotechnology, Mendel University in Brno, Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic
Rene Kizek
; Department of Chemistry and Biochemistry, Laboratory of Metallomics and Nanotechnology, Mendel University in Brno, Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic
Sažetak
Cisplatin belongs to the most widely used cytostatic drugs. The determination of the presence of the DNA-cisplatin adducts may not only signal the guanine-rich regions but also monitor the interaction reaction between DNA and the drug in terms of speed of interaction.
In this work, the combined advantages of magnetic particles-based isolation/purification with fluorescent properties of quantum dots (QDs) and antibodies targeted on specific recognition of DNA-cisplatin adducts are demonstrated. The formation of a complex between magnetic particles with surface modified by anti-dsDNA antibody, cisplatin-modified DNA and QDs labelled anti-cisplatin-modified DNA antibody was suggested and optimized.
Ključne riječi
Anti-DNA Antibodies; Cisplatin; Magnetic Separation; Sandwich Analysis
Hrčak ID:
157688
URI
Datum izdavanja:
1.1.2015.
Posjeta: 1.046 *