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The relationship between the methods for the determination of erythrocyte protoporphyrins (EP) and zinc protoporphyrins (ZPP)

Lj. Skender ; Institut za medicinska istraživanja i medicinu rada, Zagreb, Hrvatska
D. Prpić-Majić ; Institut za medicinska istraživanja i medicinu rada, Zagreb, Hrvatska
V. Karačić ; Institut za medicinska istraživanja i medicinu rada, Zagreb, Hrvatska


Puni tekst: hrvatski pdf 9.070 Kb

str. 227-237

preuzimanja: 306

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Sažetak

In order to find the most convenient method for erythrocyte protoporphyrin (EP) determination, a very important biochemical parameter in the evaluation of increased lead absorption, the relationship between the Chisolm-Brown spectrofluorometric method and the Cripps-Peters spectrophotometric method for EP determination, as well as the relationship between the two methods and direct zinc-protoporphyrin (ZPP) determination by means of a haematofluorometer were examined. A very significant connection between all the three examined methods was established by means of the correlation coefficient. From the regression line the sensitivity of the Chisolm-Brown spectrofluorometric method was found to be higher than of the Cripps-Peters method for EP. The method of direct ZPP determination also proved to be more sensitive than the Cripps-Peters spectrophotometric method. The Chisolm-Brown spectrofluorometric method was shown to be specific, acourate, sensitive and reproducible. In addition, it is relatively quick and easy to apply, does not need a large quantity of biological samples and complicated technical instruments. Because of these characteristics it is recommended as a method of choice for EP determination in adults and children for examination of increased lead absorption, sideropenic anaemia and in some genetic diseases of disturbed porphyrin synthesis.

Ključne riječi

Hrčak ID:

158491

URI

https://hrcak.srce.hr/158491

Datum izdavanja:

17.2.1983.

Podaci na drugim jezicima: hrvatski

Posjeta: 803 *