Veterinarski arhiv, Vol. 86 No. 3, 2016.
Izvorni znanstveni članak
The effect of estrogen receptor genotypes on the number of stillborn and mummified piglets in Topigs 20 sows.
Sven Menčik
; Department of Animal Husbandry, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Vlado Vuković
; Department of Livestock Production, Faculty of Agricultural Sciences and Food, Skopje, Macedonia
Mario Modrić
; Krmiva Ltd., Zagreb, Croatia
Marija Špehar
; Croatian Agricultural Agency, Zagreb, Croatia
Mario Ostović
; Department of Animal Hygiene, Behavior and Welfare, Faculty of Veterinary Medicine,Department of Animal Hygiene, Behavior and Welfare, Faculty of Veterinary Medicine,Department of Animal Hygiene, Behavior and Welfare, Faculty of Veterinary Medicine, Unive
Velimir Sušić
; Department of Animal Husbandry, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Igor Štoković
; Department of Animal Husbandry, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Krešimir Salajpal
; Department of Animal Science, Faculty of Agriculture, University of Zagreb, Zagreb, Croatia
Hrvoje Kabalin
; Jastrebarsko Veterinary Station, Pisarovina Clinic, Pisarovina, Croatia
Anamaria Ekert Kabalin
; Department of Animal Husbandry, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Sažetak
Steroid hormones such as estrogen play a central role in the postnatal female physiology and their effects are exerted through its receptors. The estrogen receptor gene is one of the first and most extensively investigated candidate genes for reproductive traits in pigs, especially for litter size. The aim of the study was to investigate the estrogen receptor gene polymorphism using endonuclease PvuII, and its association with the number of stillborn (NSB) and the number of mummified (NMUM) piglets in the first, second, third and subsequent parities and in overall parities. Topigs 20 sows (n = 101) from 1st to 7th parities were analyzed. Estrogen receptor genotypes were detected by the polymerase chain reaction-restriction fragment length polymorphism method. Two alleles (A and B) were identified with three genotypes. Alleles (A and B) and genotype frequencies were determined. Comparison of the observed and expected genotype frequencies was performed using the χ2-test and considerable deviation from the Hardy-Weinberg principle was found. All data were analyzed using the General Linear Model. Statistical analysis showed a significant difference (P<0.05) in NMUM between the AB and BB genotypes in the third and subsequent parities. The statistical significance of differences between AA and BB genotypes tended to be lower (P<0.1) in the third and subsequent parities in NMUM. The sows with A allele had less NSB, indicating a beneficial effect of this allele, unlike the B allele in NMUM. The results obtained will contribute to the understanding of the effect of ESR genotype on NSB and NMUM, and substantiate genetic evaluation of litter size traits in pigs.
Ključne riječi
estrogen; genotype; stillborn and mummified piglets; Topigs 20
Hrčak ID:
160424
URI
Datum izdavanja:
10.6.2016.
Posjeta: 896 *