Šumarski list, Vol. 140 No. 5-6, 2016.
Pregledni rad
https://doi.org/10.31298/sl.140.5-6.6
Micropropagation of wild cherry (Prunus avium L.) from a clonal seed orchard
Olivera Tančeva Crmarić
; Vitroplant Ltd., Solin, Croatia
Davorin Kajba
orcid.org/0000-0001-9194-1030
; Faculty of Forestry University of Zagreb
Sažetak
A total of 24 genotypes (selected adult plus trees) were introduced from a clonal seed orchard of wild cherry (Prunus avium L.) in the process of in vitro production. The possibilities of optimizing routine micropropagation methods of the clones at all stages were explored. Several techniques were established that allow the introduction of the initial culture throughout the year. A total of 23 clones were successfully introduced over the four seasons. Clone L3 was not successfully disinfected and was not introduced into the initial culture. The specific composition of culture media and the unique combination of growth regulators were determined in all in vitro production stages, leading to the growth of high-quality plants with very good survival during the acclimatization process. BAP-1.0 mg/L, Kinetin-0.5 mg/L and IAA-0.5 mg/L were used for micropropagation, which resulted in the multiplication rate of 3-9 with shoot heights from 1.5 to 3.0 cm. Rooting of microcuttings was achieved by a combination of IAA-2.0 mg/L, IBA-2.0 mg/L with the addition of GA3-0.2 mg/L. The rooted plantlets of wild cherry developed normal internodes and leaf blades, and the roots were well formed with 3 to 7 roots per plant, whose length increased the longer they remained in the culture medium. The micropropagated selected plus trees manifested strong apical dominance and the majority of the young plants reached a height of over one meter in the period of seven months.
Ključne riječi
<i>in vitro</i> propagation; selected plus trees; clonal seed orchard; wild cherry
Hrčak ID:
161588
URI
Datum izdavanja:
30.6.2016.
Posjeta: 3.055 *