Pregledni rad

https://doi.org/10.11613/BM.2016.026

Haemoglobin J-Baltimore can be detected by HbA1c electropherogram but with underestimated HbA1c value.

Valéry Brunel ; Department of Medical Biochemistry, Rouen University Hospital, Rouen, France
Agnes Lahary ; Department of Haematology, Rouen University Hospital, Rouen, France
Abdeslam Chagraoui ; Department of Medical Biochemistry, Rouen University Hospital, Rouen, France
Christian Thuillez ; Department of Medical Biochemistry, Rouen University Hospital, Rouen, France

Sažetak

Glycated haemoglobin (HbA1c) is considered the gold standard for assessing diabetes compensation and treatment. In addition, fortuitous detection of haemoglobin variants during HbA1c measurement is not rare. Recently, two publications reported different conclusions on accuracy of HbA1c value using capillary electrophoresis method in presence of haemoglobin J-Baltimore (HbJ).
Here we describe the fortuitous detection of unknown HbJ using capillary electrophoresis for measurement of HbA1c. A patient followed for gestational diabetes in our laboratory presented unknown haemoglobin on Capillarys 2 Flex Piercing analyser which was identified as HbJ. HbJ is not associated with haematological abnormalities. High Performance Liquid Chromatography methods are known to possibly underestimate HbA1c value in the presence of this variant. This variant and its glycated form are clearly distinguished on electropherogram but HbJ was responsible for underestimating the true area of HbA1c.
Capillary electrophoresis is a good method for detecting HbJ but does not seem suitable for evaluation of HbA1C value in patients in presence of HbJ variant.

Ključne riječi

glycated haemoglobin; haemoglobin J-Baltimore; capillary electrophoresis

Hrčak ID:

161693

URI

https://hrcak.srce.hr/161693

Datum izdavanja:

15.6.2016.

Posjeta: 1.000 *