Poljoprivreda, Vol. 13 No. 1, 2007.
Izvorni znanstveni članak
THE IMPACT OF PROMOTERS AND CELL-LINE TYPES ON ALTERNATIVE SPLICING OF RECOMBINANT CONSTRUCTS
Polona Frajman
P. Dovč
Sažetak
Alternative intron splicing is a process where introns are excised from pre-mRNA following different patterns. The consequence of it is a presence of several different forms of mature mRNAs differing in length and in content of the whole exons or their parts. Studies in the past decade lead to the view that a complex and extensively coupled network has evolved to coordinate the activities of gene expression pathway, which includes transcription, several pre-mRNA processing steps and the export of mature mRNA to the cytoplasm. Coupling of transcription to splicing was revealed in studies showing that transcription of pre-mRNA by different promoters can generate different alternatively spliced mRNAs. The main goal of this study was to determine the impact of different milk gene promoters on alternative splicing under in vitro conditions. Besides, we also investigated how different cell types influence the splice site selection. With this experiment, we confirmed our predictions that promoter structure and cell-type have an impact on alternative splicing. Differences were observed in quantity of transcripts, as well as in splice-site selection.
Ključne riječi
alternative splicing; milk-protein gene promoters; cell lines
Hrčak ID:
16058
URI
Datum izdavanja:
29.6.2007.
Posjeta: 1.136 *