Izvorni znanstveni članak

Efficacy of different extenders on sperm characteristics and fertility in crossbred pigs of north-eastern India.

Kadirvel Govindasamy ; Division of Animal Production, ICAR- Research Complex for NEH Region, Umiam, Meghalaya, India
Perumal Ponraj ; Animal Reproduction Division, ICAR-National Research Centre on Mithun, Jharnapani, Nagaland, India
Sarath Thulasiraman ; Department of Clinics, ICAR-National Research Centre on Mithun, Madras Veterinary College, Chennai, India
Jerome Andonissamy ; ICAR-Central Institute for Research on Buffaloes, Hisar, Haryana, India
Syamal Naskar ; Division of Animal Production, ICAR- Research Complex for NEH Region, Umiam, Meghalaya, India
Anubrata Das ; Division of Animal Production, ICAR- Research Complex for NEH Region, Umiam, Meghalaya, India
Dhilruba Hasin ; Division of Animal Production, ICAR- Research Complex for NEH Region, Umiam, Meghalaya, India
Santosh K. Baishya ; Division of Animal Production, ICAR- Research Complex for NEH Region, Umiam, Meghalaya, India

Sažetak

The study was conducted to deduce the efficacy and suitability of three extenders: Beltsville thawing solution (BTS), Kiev and Modena extenders, on the preservation and fertility of boar semen. Semen ejaculates with more than 70% sperm motility from six Hampshire boars were used and preserved at 17 °C. The extended semen was evaluated for motility, viability, acrosomal and plasma membrane integrity from day 0 to 5 of preservation. There was a significant (P<0.01) reduction in sperm motility and viability between days 0 to 4, but no significant difference between day 0 and 2. BTS and Modena maintained significantly (P<0.05) higher sperm motility and viability as compared to Kiev. Plasma membrane integrity did not differ significantly up to day 2, but was significantly reduced on days 3 (P<0.05) and 4 (P<0.01). BTS and Modena extenders had significantly (P<0.05) higher plasma membrane integrity. By day 5, the percentage of mean sperm with intact acrosome was 80.4 ± 2.5, 81.2 ± 2.3 and 75.7 ± 2.7, in BTS, Modena and Kiev, respectively, with significant (P<0.05) increases in acrosomal damage after two days of storage, irrespective of all extenders. Farrowing rate and litter size did not differ significantly during the initial period of storage, but there was a significant difference after day 3 (P<0.05) in all extenders used. BTS and Modena maintained a farrowing rate of more than 60% on day 5, in contrast to 50% with the Kiev extender. The study concluded that both BTS and Modena are better extenders for short term storage of boar liquid semen as compared to the Kiev extender, and could be used efficiently in crossbreeding of pigs of north-eastern India.

Ključne riječi

extenders; liquid boar semen; sperm motility; membrane integrity; farrowing rate; litter size

Hrčak ID:

165727

URI

https://hrcak.srce.hr/165727

Datum izdavanja:

12.8.2016.

Podaci na drugim jezicima: hrvatski

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