Izvorni znanstveni članak

https://doi.org/10.5562/cca2832

A Tyrosine-Dependent Riboswitch Controls the Expression of a Tyrosyl-tRNA Synthetase from Acidithiobacillus ferrooxidans

Paula Bustamante ; Program of Cellular and Molecular Biology, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile, Santiago, 8380453, Chile
Oriana Salazar ; Centre for Biochemical Engineering and Biotechnology, Department of Chemical Engineering and Biotechnology, Faculty of Physical Sciences and Mathematics, University of Chile, Santiago, Chile
Héctor Garcías-Papayani ; Program of Cellular and Molecular Biology, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile, Santiago, 8380453, Chile
Pamela Alamos ; Program of Cellular and Molecular Biology, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile, Santiago, 8380453, Chile
Omar Orellana ; Program of Cellular and Molecular Biology, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile, Santiago, 8380453, Chile

Sažetak

Expression of aminoacyl-tRNA synthetases is regulated by a variety of mechanisms at the level of transcription or translation. A T-box dependent transcription termination / antitermination riboswitch system that responds to charged / uncharged tRNA regulates expression of aminoacyl tRNA synthetase genes in Gram-positive bacteria. TyrZ, the gene encoding tyrosyl-tRNA synthetase from Acidithiobacillus ferrooxidans, a Gram-negative acidophilic bacterium that participates in bioleaching of minerals, resembles the gene from Bacillus subtilis including the 5´-untranslated region encoding the riboswitch. Transcription of A. ferrooxidans tyrZ is induced by the presence of tyrosine by a mechanism involving antitermination of transcription. This mechanism is probably adapted to the low supply of amino acids of acidic environments of autotrophic bioleaching microorganisms.

This work is licensed under a Creative Commons Attribution 4.0 International License.

Ključne riječi

riboswitch; T-box; TyrRS; <i>acidophilic bacterium</i>; tyrosine

Hrčak ID:

166014

URI

https://hrcak.srce.hr/166014

Datum izdavanja:

21.6.2016.

Posjeta: 2.455 *