Izvorni znanstveni članak
https://doi.org/10.3325/cmj.2016.57.151
Putative anticancer potential of novel 4-thiazolidinone derivatives: cytotoxicity toward rat C6 glioma in vitro and correlation of general toxicity with the balance of free radical oxidation in rats
Lesya I. Коbylinska
; Department of Biochemistry, Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
Nataliya M. Boiko
; Department of Regulation of Cell Proliferation and Apoptosis,Institute of Cell Biology, NAS of Ukraine, Lviv, Ukraine
Rostyslav R. Panchuk
; Department of Regulation of Cell Proliferation and Apoptosis,Institute of Cell Biology, NAS of Ukraine, Lviv, Ukraine
Iryna I. Grytsyna
; Department of Regulation of Cell Proliferation and Apoptosis,Institute of Cell Biology, NAS of Ukraine, Lviv, Ukraine
Olga Yu. Klyuchivska
; Department of Regulation of Cell Proliferation and Apoptosis,Institute of Cell Biology, NAS of Ukraine, Lviv, Ukraine
Liliya P. Biletska
; Department of Biochemistry, Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
Roman B. Lesyk
; Department of Pharmaceutical Chemistry, Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
Borys S. Zіmenkovsky
; Department of Pharmaceutical Chemistry, Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
Rostyslav S. Stoika
; Department of Regulation of Cell Proliferation and Apoptosis,Institute of Cell Biology, NAS of Ukraine, Lviv, Ukraine
Sažetak
Aim To evaluate the cytotoxic action of 4-thiazolidinone derivatives
(ID 3288, ID 3882, and ID 3833) toward rat glioma
C6 cells and to compare the effects of these compounds
and doxorubicin on the balance of free radical oxidation
(FRO) and antioxidant activity (AOA) in the serum of rats.
Methods Glioma cells were treated with ID 3882, ID 3288,
ID 3833, and doxorubicin, and their cytotoxicity was studied
using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide) assay and Trypan blue exclusion test, light
and fluorescent microscopy, and flow cytometric study of
cell cycling and apoptosis, including measuring of Annexin
V-positive cells. The contents of superoxide radical, hydrogen
peroxide, hydroxyl radical, malonic dialdehyde, and hydrogen
sulfide were measured in the serum of rats. Enzymatic
activity of superoxide dismutase (SOD), catalase (Cat),
and glutathione peroxydase (GPO) was determined.
Results Among novel 4-thiazolidinone derivatives, ID 3288
was most toxic toward rat glioma C6 cells, even compared
with doxorubicin. All applied derivatives were less active
than doxorubicin in inducing reactive oxygen species-related
indicators in the serum of rats. A similar effect was observed
when enzymatic indicators of AOA processes were
measured. While doxorubicin inhibited the activity of SOD,
GPO, and Cat, the effects of 4-thiazolidinone derivatives
were less prominent.
Conclusion Novel 4-thiazolidinone derivatives differ in their
antineoplastic action toward rat glioma C6 cells, and ID 3288
possesses the highest activity compared to doxorubicin.
Measurement of indicators of FRO and AOA in the serum of
rats treated with these compounds showed their lower general
toxicity compared with doxorubicin’s toxicity.
Ključne riječi
Hrčak ID:
169491
URI
Datum izdavanja:
15.4.2016.
Posjeta: 1.071 *