Izvorni znanstveni članak

Alterations of hypothalamic RFamide related peptide-3 and Kiss1 gene expressions during spermatogenesis of rat in chronic stress conditions.

Maryam Karami Kheirabad ; Department of Basic Sciences, Azad University, Gachsaran branch, Gachsaran, Iran
Somayeh Ahmadloo ; Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Bahia Namavar Jahromi ; Infertility Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Farhad Rahmanifar ; Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
Amin Tamadon orcid.org/0000-0002-0222-3035 ; Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Amin Ramezani ; Department of Medical Biotechnology, School of Advanced Medical Sciences and Technology, Shiraz University of Medical Sciences, Shiraz, Iran; Institute of Cancer Research, Shiraz University of Medical Sciences, Shiraz, Iran
Maryam Owjfard ; Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Fatemeh Sabet Sarvestani ; Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Omid Koohi-Hosseinabadi ; Center of Comparative and Experimental Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

Sažetak

The effects were evaluated of chronic stress and the glucocorticoid receptor antagonist (RU486) on mRNA expressions of RF-amide related peptide-3 (RFRP-3) in the dorsomedial hypothalamic nucleus (DMH) and Kiss1 in the arcuate nucleus (ARC) of male rats. Twenty-four male rats were allocated to four equal sized groups: the stress, RU486, stress/RU486, and control groups. In the stress group the rats were restrained 1 hour/day for 12 days. In the RU486 group, the rats were injected with RU486 for 12 days. In the stress/RU486 group, the rats were injected with RU486 1 hour before the stress process for 12 days. Relative expressions of RFRP-3 and Kiss1 mRNAs were determined using real-time PCR. The relative expression of RFRP-3 mRNA in the stress group was higher than that in the RU486 and control rats. The relative expression of RFRP-3 mRNA did not differ between the stress group and the stress/RU486 rats. Furthermore, the relative expressions of Kiss1 mRNA in the stress, RU486, and stress/RU486 groups were less than that of the control rats. The relative expression of Kiss1 mRNA did not differ between the stress, RU486, and stress/RU486 groups. In conclusion, dysfunction in male rat fertility caused by the chronic stress may be the result of the increase in REFP-3 and the decrease in Kiss1 mRNA expression.

Ključne riječi

chronic stress; RFRP-3; kisspeptin; hypothalamus; rats

Hrčak ID:

170225

URI

https://hrcak.srce.hr/170225

Datum izdavanja:

22.11.2016.

Podaci na drugim jezicima: hrvatski

Posjeta: 1.856 *