Pregledni rad
Role of Law Enforcement Response and Microbial Forensics in Investigation of Bioterrorism
Bruce Budowle
Jodi A. Beaudry
Neel G. Barnaby
Alan M. Giusti
Jason D. Bannan
Paul Keim
Sažetak
The risk and threat of bioterrorism and biocrime have become a
large concern and challenge for governments and society to enhance
biosecurity. Law enforcement plays an important role in assessing
and investigating activities involved in an event of bioterrorism
or biocrime. Key to a successful biosecurity program is increased
awareness and early detection of threats facilitated by an integrated
network of responsibilities and capabilities from government,
academic, private, and public assets. To support an investigation,
microbial forensic sciences are employed to analyze and characterize
forensic evidence with the goal of attribution or crime scene reconstruction.
Two different molecular biology-based assays – real
time polymerase chain reaction (PCR) and repetitive element PCR
– are described and demonstrate how molecular biology tools may
be utilized to aid in the investigative process. Technologies relied
on by microbial forensic scientists need to be properly validated so
that the methods used are understood and so that interpretation of
results is carried out within the limitations of the assays. The three
types of validation are preliminary, developmental, and internal.
The first is necessary for rapid response when a threat is imminent or
an attack has recently occurred. The latter two apply to implementation
of routinely used procedures.
Ključne riječi
bioterrorism; biocrime, microbial forensics; law enforcement; real time PCR; repetitive element PCR; validation; genetics; molecular biology
Hrčak ID:
16583
URI
Datum izdavanja:
15.8.2007.
Posjeta: 2.656 *